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Rapid Rat Antibody Isotyping Test Kit

Rat Antibody Isotyping Kit plus Kappa and Lambda, Rat Monoclonal Antibody Isotyping Kit, Rat Hybridoma Isotyping Kit, Rat mAb Isotyping Test Kit

Catalog No. Product Name Size List Price (US$) Quantity
EK0303 Rapid Rat Monoclonal Antibody Isotyping Test Kit 10 tests 220.00
Description

Background

A typical immunoglobulin (antibody) molecule is composed of four polypeptide chains, two identical heavy chains and two identical light chains. In rats, based on the heavy chain constant regions, antibodies are classified into IgA, IgD, IgE, IgG, and IgM, and the IgG isotype is further divided into IgG1, IgG2a, IgG2b, and IgG2c; the light chain constant regions are classified into kappa (κ) and lambda (λ).  The IgG isotypes share more than 95% homology in the amino acid sequences of the Fc regions but show major differences in the amino acid composition and structure of the hinge region. Murine kappa/lambda ratio is 95/5, significantly different from the human kappa/lambda ratio of 60/40.

The isotype and subclass for a rat antibody can be determined by specific anti-heavy or light chain constant region antibodies, usually through lateral flow in-vitro immunoassay or ELISA, with appropriate isotype controls. We have developed rapid antibody isotyping test kits to determine the isotypes of antibody heavy and light chains as rapidly, conveniently, and accurately as many pregnancy test kits.

EK0303: Rapid Rat Monoclonal Antibody Isotyping Test Kit

The rapid rat antibody isotyping test kit is a five-minute lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. It can be used to determine the isotype of IgG1, IgG2a, IgG2b, IgG2c, IgA, and IgM, as well as kappa and lambda in one test kit. There are two cassettes in the test kit, one for IgG1, IgG2a, IgG2b, and IgG2c, and the other for kappa, Lamda, IgA, and IgM.
Sensitivity: Minimal detection limit is 100 ng/ml.
Specificity: There is no cross-reactivity among each isotype.
Sample requirements: hybridoma culture supernatant and purified monoclonal antibody. Although ascites fluid is not recommended since there is usually multiple immunoglobulins, the kit can be used to determine the relative distribution of the main isotypes in the ascites fluid.

Sample preparation:
Cell culture supernatant: 1:10~1:100 diluted in PBS.
Purified Antibody: 0.5 – 2 µg/mL in PBS.
Ascites fluid: 1:10,000 diluted in PBS.

Protocol of the rapid rat antibody isotyping test kit:
1) Place the cassettes in horizontal plane.
2) Add 80-100 µL antibody solution directly to the sample well. Wait for 5 minutes.
3) A red test line at the location corresponding to antibody-specific isotype and a red control line at “C" position will appear within 1-5 minutes.

Result interpretation:
1. Invalid result: no red line at “C”.
2. Negative result: only one red line at “C”.
3. Positive results:
a). Single monoclonal antibody: In addition to the positive control “C” line, only one heavy chain and one light chain will appear.
b). Multiple monoclonal antibodies: In addition to the positive control “C” line, more than one heavy chain and/or light chain will appear. The line intensity can be used to determine the dominant isotypes.

Shipping: The rapid rat antibody isotyping test kit is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the strips in their original package at room temperature. This product is stable for up to 18 months from the certified date. Freezing the strips is not recommended.

Other rapid antibody isotyping test kits:
Rapid Human Monoclonal Antibody Isotyping Test Kit
Rapid Hamster Monoclonal Antibody Isotyping Test Kit
Rapid Mouse Monoclonal Antibody Isotyping Test Kit A
Rapid Mouse Monoclonal Antibody Isotyping Test Kit C
Rapid Mouse Monoclonal Antibody Isotyping Test Kit A+C

Recombinant rat IgG isotype control antibodies:
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Rat IgG Isotype Control Antibodies
In vivo Grade Recombinant HyHEL-10 Rat IgG Isotype Control Antibodies
In vivo Grade Recombinant HEL D1.3 Rat IgG Isotype Control Antibodies
In vivo Grade Recombinant C1.18.4 Rat IgG Isotype Control Antibodies
In vivo Grade Recombinant B1-8 Rat IgG Isotype Control Antibodies

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