BP004987: Biotinylated Human PD-1/PDCD1 Protein with the C-His-Avi Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated human PD-1 with the His-Avi Tag with an affinity constant of 2.356 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005587: Biotinylated Human PD-1/PDCD1 Protein with the C-hFc-Avi Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated recombinant human PD-1/PDCD1 protein with the hFc-Avi tag to the immobilized human PD-L1 with the mFc tag at 2 ug/ml (100 ul/Well) was 0.19 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005136: Mouse PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant mouse PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant mouse PD-L1 with the C-His Tag captured on CM5 Chip via anti-His antibody can bind to the recombinant mouse PD-1 with the C-hFc Tag with an affinity constant of 0.62 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.PA000123-R12-2432: ABL1 Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB, IHC, ELISA;
Reactivity: Human, Mouse, Rat.IA123: NMab Protein G Resin
* Cost competitive, high binging capacity alkaline-stable protein G resins.
* High dynamic binding capacity: 55 - 65 mg/mL (human IgG, 5 minute residence time).
* Clean in Place: 6 M guanidine hydrochloride.
BP003700: Cynomolgus PD-1/PDCD1 Protein with the C-His Tag
Source: The recombinant cynomolgus PD-1/PDCD1 protein, the PD1 ECD (amino acids Pro21-Gln167) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated anti-cynomolgus PD-1 antibody with the hFc tag to the immobilized cynomolgus PD-1/PDCD1 protein with the His tag at 2 ug/ml (100 ul/Well) was 4.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005346: Cynomolgus PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant cynomolgus PD-1/PDCD1 protein, the PD1 ECD (amino acids Pro21-Gln167) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated anti-cynomolgus PD-1 antibody with the hFc tag to the immobilized cynomolgus PD-1/PDCD1 with the C-hFc tag at 0.5 ng/ml (100 ul/Well) was 29.5 ng/ml determined by ELISA. The recombinant cynomolgus PD-1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated recombinant cynomolgus PD-L1 with the C-His-Avi Tag with an affinity constant of 1.28 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003202: Canine PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant canine PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Val170) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the recombinant human PD-L1 protein with the mFc tag to the immobilized canine PD-1/PDCD1 with the C-hFc tag at 1 ug/ml (100 ul/Well) was 30.7 ng/ml determined by ELISA. The recombinant canine PD-1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-L1 with the C-His Tag with an affinity constant of 0.696 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005447: Human PD-1/PDCD1 Protein with the C-His Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Activity: The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the recombinant human PD-1 with the C-His Tag with an affinity constant of 2.2 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005354: Human PD-1/PDCD1 Protein with the C-hFc Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the recombinant human PD-L1 protein with the mFc tag to the immobilized human PD-1/PDCD1 with the C-hFc tag at 1 ug/ml (100 ul/Well) was 0.1 ug/ml determined by ELISA. The recombinant human PD-1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated recombinant human PD-L2 with the C-His-Avi Tag with an affinity constant of 0.344 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005376: Human PD-1/PDCD1 Protein with the C-mFc Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a mFc at the C-terminus, was produced in HEK 293 cells.
Activity: The EC50 of the biotinylated anti-human PD-1 antibody with the hFc tag to the immobilized human PD-1/PDCD1 protein with the mFc tag at 0.5 ug/ml (100 ul/Well) was 68 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.