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Mouse PD-1 Protein

Programmed death-1, CD279, PDCD1, Programmed Death-1 Receptor

Catalog No. Product Name Size List Price (US$) Quantity
BP005136 Mouse PD-1/PDCD1 Protein with the C-hFc Tag 100 ug 300.00
BP005136 Mouse PD-1/PDCD1 Protein with the C-hFc Tag 500 ug 900.00
Description

BP005136: Mouse PD-1/PDCD1 Protein with the C-hFc Tag

Source: The recombinant mouse PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant mouse PD-1/PDCD1 protein with the C-hFc tag has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity: The recombinant mouse PD-L1 with the C-His Tag captured on CM5 Chip via anti-His antibody can bind to the recombinant mouse PD-1 with the C-hFc Tag with an affinity constant of 0.62 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant mouse PD-1/PDCD1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping: The recombinant mouse PD-1/PDCD1 protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins

PD1 and PDL1 Antibodies:
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)

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