BP004856: Biotinylated Human VEGF-C/Flt4-L Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human VEGF-C/Flt4-L Protein, (amino acids Thr103-Arg227) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human VEGF-C protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human VEGF R3 protein with the C-hFc tag with the EC50 of 8.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005126: Human VEGF-C/Flt4-L Protein with the C-His-Avi Tag
Source: The recombinant Human VEGF-C/Flt4-L Protein, (amino acids Thr103-Arg227) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human VEGF-C protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human VEGF R3 protein with the C-hFc tag with the EC50 of 17.9 ng/ml determined by ELISA (QC Test). The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000258-CYT-262: Recombinant Rat Vascular Endothelial Growth Factor C (VEGF-C) Protein
Source: Sf9, Insect Cells-derived.
Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells. The ED50 for this effect is typically 200-300 ug/ml corresponding to a Specific Activity of 3,334-5,000 IU/mg.
Purity: > 90% by SDS-PAGE and RP-HPLC.BP000258-CYT-284: Recombinant Rat Vascular Endothelial Growth Factor C (VEGF-C) Protein
Source: Sf9, Insect Cells-derived.
Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT -4 cells). The ED50 for this effect is typically 150-300 ug/ml, corresponding to a specific activity of 3.3-6.6 KUnits/mg.
Purity: > 90% by SDS-PAGE and RP-HPLC.BP000258-CYT-527: Recombinant Human Vascular Endothelial Growth Factor C (VEGF-C) Protein
Source: Sf9, Insect Cells-derived.
Measured by its ability to bind to the VEGFR-3/FLT-4 receptor in the Ba/F3-hVEGFR-3 assay. The ED50 in this assay is about 4 ug/ml corresponding to a Specific Activity of 250,000 IU/mg.
Purity: > 90% by SDS-PAGE and RP-HPLC.