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- Normal-phase chromatography (NP-HPLC) is an analytical technique that is used to separate molecules on the basis of differences in the strength of their interaction with a polar stationary phase.
Sample requirement: 100-200 ug.
Fast turnaround time: 2 - 3 weeks. Peptide mapping by LC-MS/MS can be used to determine detailed information about the peptide's sequence and post-translational modifications.
- Reversed-phase chromatography (RP-HPLC) is an analytical technique that separates molecules on the basis of differences in their hydrophobicity.
Sample requirement: 50-100 ug.
Fast turnaround time: 1 - 2 weeks.
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