PA007486.r2a: Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) Monoclonal Antibody (Clone: 3H3), Rat IgG2a Kappa

Recombinant rat IgG2a monoclonal antibody.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3H3 antibody specifically binds to mouse 4-1BB protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).

PA007486.h1Fs: Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) Monoclonal Antibody (Clone: 3H3), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with Fc silent mutation.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3H3 antibody specifically binds to mouse 4-1BB protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).

PA007715.h1Fs-b: Biotinylated Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) Monoclonal Antibody (Clone: 3H3), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain. 
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 3H3 antibody specifically binds to mouse 4-1BB protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.

PA007380.m2b: In vivo Grade Recombinant Anti-mouse CTLA-4 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: 9D9)

Recombinant Mouse IgG2b Monoclonal Antibody. 
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 9D9) specifically binds to the mouse CTLA-4 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CTLA-4 protein.

PA007276.r2a: In vivo Grade Recombinant Anti-mouse CD137 (TNFRSF9 or 4-1BB) Monoclonal Antibody, Rat IgG2a Kappa (Clone: 3H3)

Recombinant rat IgG2a Monoclonal Antibody. 
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 3H3) specifically binds to the mouse 4-1BB protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse 4-1BB protein.

PA007545.m2a: In Vivo Grade Recombinant Anti-mouse CTLA-4 (CD152) Monoclonal Antibody (Clone: UC10-4F10-11), Mouse IgG2a Kappa

Recombinant mouse IgG2a Monoclonal Antibody.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: UC10-4F10-11) specifically binds to mouse CTLA-4 (CD152).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CTLA-4 (CD152) protein.

PA007545.hs: In Vivo Grade Recombinant Anti-mouse CTLA-4 (CD152) Monoclonal Antibody (Clone: UC10-4F10-11), Armenian hamster IgG

Recombinant hamster IgG Monoclonal Antibody.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: UC10-4F10-11) specifically binds to mouse CTLA-4 (CD152).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CTLA-4 (CD152) protein.

PA007525.m2a: In Vivo Grade Recombinant Anti-mouse NK1.1 Monoclonal Antibody (Clone: PK136), Mouse IgG2a Kappa

Recombinant mouse IgG2a Monoclonal Antibody.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: PK136) specifically binds to mouse NK1.1.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse NK1.1 protein.

Experienced scientists working on antigen and antibody production, antibody engineering, lentivirus, and cell line development services for decades. >90% success rate of stable cell line generation.

Fast turnaround:

Antigen (peptide or protein) production: 3-6 weeks.
Mouse monoclonal antibody production: 20-24 weeks.
Screening phage or yeast libraries for fully human antibodies: 8-12 weeks.
Sequencing hybrioma antibody: 1-3 weeks.
Chimeric antibody production: 3-4 weeks.
Antibody humanization: 8-10 weeks.
Construction of CAR cassette, lentivirus packaging and production: 4-5 weeks.
T-cell activation, transduction, and CAR T-cell expansion: 4-5 weeks.
Confirmation of CAR expression, IFN-gamma ELISA, and cytotoxicity assay of transduced and expanded T-cells: 2 weeks.

PA007168.m2a: In Vivo Grade Recombinant Anti-Mouse OX40 Mouse IgG2a Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse OX40 mouse IgG2a monoclonal antibody (clone of OX86) was produced in mammalian cells.
Applications: ELISA, Western blot (WB), immunofluorescence (IF), Flow Cytometry (FC), and various in vitro and in vivo functional assays.

PA007168.r1: In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG1 Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse OX40 rat IgG1 monoclonal antibody (clone of OX86) was produced in mammalian cells.
Applications: ELISA, Western blot (WB), immunofluorescence (IF), Flow Cytometry (FC), and various in vitro and in vivo functional assays.

BP005340: Human OX40/TNFRSF4/CD134 Protein with the C-hFc Tag

Source: The recombinant Human OX40/TNFRSF4/CD134 Protein, (amino acids Leu29-Ala216) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human OX40 Ligand Trimer protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human OX40 protein with the C-hFc tag with the EC50 of 37.7 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005429: Human OX40/TNFRSF4/CD134 Protein with the C-His-Avi Tag

Source: The recombinant Human OX40/TNFRSF4/CD134 Protein, (amino acids Leu29-Ala216) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005210: Biotinylated Human OX40/TNFRSF4/CD134 Protein with the C-hFc Tag

Source: The recombinant Biotinylated Human OX40/TNFRSF4/CD134 Protein, (amino acids Leu29-Ala216) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005613: Biotinylated Human OX40/TNFRSF4/CD134 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human OX40/TNFRSF4/CD134 Protein, (amino acids Leu29-Ala216) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human OX40 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human OX40 Ligand protein with the C-hFc tag with the EC50 of 3.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004284: Mouse OX40/TNFRSF4/CD134 Protein with the C-His-Avi Tag

Source: The recombinant Mouse OX40/TNFRSF4/CD134 Protein, (amino acids Val20-Pro211) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse OX40 Ligand protein with the C-His-Avi tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse OX40 protein with the C-His tag with the EC50 of 1.06 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.