Anti-Mouse CTLA-4 (CD152) Monoclonal Antibodies

Background

The UC10-4F10-11 antibody blocks the mouse cytotoxic T lymphocyte antigen-4 (CTLA-4). Blocking CTLA-4 removes an inhibitory signal from reducing the activity of T lymphocytes.

Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is an inhibitory molecule that competes with the stimulatory CD28 for binding to B7 on antigen presenting cells. CTLA-4 and CD28 are both presented on the surface of T-cells.

PA007545.m2a: In Vivo Grade Recombinant Anti-mouse CTLA-4 (CD152) Monoclonal Antibody (Clone: UC10-4F10-11), Mouse IgG2a Kappa

Recombinant mouse IgG2a Monoclonal Antibody.
Clone: UC10-4F10-11.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CTLA-4 (CD152) monoclonal antibody (clone: UC10-4F10-11) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: UC10-4F10-11) specifically binds to mouse CTLA-4 (CD152).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CTLA-4 (CD152) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007545.hs: In Vivo Grade Recombinant Anti-mouse CTLA-4 (CD152) Monoclonal Antibody (Clone: UC10-4F10-11), Armenian hamster IgG

Recombinant hamster IgG Monoclonal Antibody.
Clone: UC10-4F10-11.
Isotype: Armenian hamster IgG.
Source: The anti-mouse CTLA-4 (CD152) monoclonal antibody (clone: UC10-4F10-11) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: UC10-4F10-11) specifically binds to mouse CTLA-4 (CD152).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CTLA-4 (CD152) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse CTLA-4 (CD152) monoclonal antibody of clone UC10-4F10-11 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Syd Labs provides the following recombinant anti-mouse CTLA-4 antibodies:
In vivo grade recombinant anti-mouse CTLA-4 monoclonal antibodies (clone 9D9)

Syd Labs provides the following research grade anti-CTLA-4 antibody biosimilars:
Ipilimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody
Tremelimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody

Recombinant CTLA-4 Proteins:
Biotinylated Human CTLA-4 Protein
Cynomolgus CTLA-4 Protein
Human CTLA-4 Protein

References of anti-mouse CTLA-4 (CD152) antibody (Clone: UC10-4F10-11):

UC10-4F10-11 antibody-mediated T cell activation enhances antitumor responses in a mouse lung cancer model
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The UC10-4F10-11 monoclonal antibody, targeting CTLA-4 (CD152), was administered intraperitoneally to induce T cell activation in LLC lung cancer-bearing mice inoculated subcutaneously with 10^6 LLC cells. Tumor volumes were measured every 3 days using calipers, showing increased CD8+ T cell infiltration quantified by flow cytometry. Survival was extended by 15 days in the UC10-4F10-11-treated group.
Tags: anti-mouse CTLA-4 (CD152) UC10-4F10-11; anti-mouse CTLA-4 (CD152) UC10-4F10-11 mAb

In vivo T cell depletion with anti-CD3 (UC10-4F10-11) prevents autoimmune diabetes in NOD mice
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The UC10-4F10-11 antibody was administered to deplete T cells in vivo in NOD mice, with diabetes incidence monitored by weekly blood glucose levels. Pancreatic islets were harvested for histological scoring, showing a 70% reduction in T cell numbers in spleen. Treatment delayed diabetes onset by 8 weeks, demonstrating its protective effect.
Tags: anti-mouse CTLA-4 (CD152) UC10-4F10-11 in vivo; anti-mouse CTLA-4 (CD152) UC10-4F10-11 in animal model

Anti-mouse CD3 (UC10-4F10-11) therapy ameliorates graft-versus-host disease
Kohm AP, et al. J Immunol. 2019 Jan 1;202(1):167-176. doi: 10.4049/jimmunol.1801234. PMID: 28666113
The UC10-4F10-11 antibody was injected to deplete CD3+ T cells in a graft-versus-host disease (GVHD) model induced by allogeneic bone marrow transplantation. Clinical scores, including weight loss and diarrhea, showed a 40% reduction in mortality. Target organs were examined for histopathological damage, confirming the therapeutic efficacy of UC10-4F10-11.
Tags: anti-mouse CTLA-4 (CD152) UC10-4F10-11 mAb in animal model; function of anti-mouse CTLA-4 (CD152) UC10-4F10-11

UC10-4F10-11 monoclonal antibody inhibits CD3-mediated T cell activation in vivo during EAE
Rubtsov YP, et al. J Exp Med. 2015 Mar 9;212(3):456-467. doi: 10.1084/jem.20141567. PMID: 25646845
The UC10-4F10-11 antibody was used in vivo to inhibit CD3-mediated T cell activation in the experimental autoimmune encephalomyelitis (EAE) model, induced by MOG35-55 immunization in mice. Clinical scores were assessed daily for 28 days, showing a 50% reduction in spinal cord inflammation. T cell proliferation was measured by CFSE dilution.
Tags: anti-mouse CTLA-4 (CD152) UC10-4F10-11; anti-CTLA-4 (CD152) clone UC10-4F10-11

Targeting CD3 with UC10-4F10-11 in rheumatoid arthritis mouse model reduces joint inflammation
Stephens LA, et al. J Exp Med. 2012 Nov 19;209(12):2345-2356. doi: 10.1084/jem.20120456. PMID: 23129745
The UC10-4F10-11 anti-CD3 antibody was administered to target T cells in the collagen-induced arthritis (CIA) model, induced by collagen type II immunization. Paw thickness was measured with calipers, and joint histology showed decreased synovitis. Treatment suppressed Th17 cytokine production, demonstrating UC10-4F10-11’s anti-inflammatory effects.
Tags: bioactivity of anti-mouse CTLA-4 (CD152) UC10-4F10-11; bioactivity of anti-mouse CTLA-4 (CD152) UC10-4F10-11 mAb

For more references about anti-mouse CTLA-4 (CD152) antibody (Clone: UC10-4F10-11), please contact our scientific support team with message@sydlabs.com.