MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
* Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
* Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
* Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
* Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.PA007144: In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
The in vivo grade recombinant rat IgG2b isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for rat IgG2b kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.BP003052: Recombinant SARS-CoV-2 Spike RBD-His-Avi
Source: mammalian cells.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.BP002388-Z53: Recombinant Human Cellular tumor antigen p53 (p53) Protein
Source: Baculovirus, SF9 insect cells-derived.
Bioactivity test: 1 unit equals 1 nanogram of purified protein. 10 unit is sufficient for a gel mobility shift assay in a 20 ul reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity: > 95%, by SDS-PAGE.
Endotoxin Level: < 1.0 EU per 1 ug of the protein by the LAL method.BP002388-Z53: Recombinant Human Cellular tumor antigen p53 (p53) Protein
Source: Baculovirus, SF9 insect cells-derived.
Bioactivity test: 1 unit equals 1 nanogram of purified protein. 10 unit is sufficient for a gel mobility shift assay in a 20 ul reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity: > 95%, by SDS-PAGE.
Endotoxin Level: < 1.0 EU per 1 ug of the protein by the LAL method.BP002273-PRO-688: Recombinant Human Solute Carrier Family 4, Sodium Bicarbonate Cotransporter, Member 4 (SLC4A4) Protein
Source: E. coli-derived.
Purity: > 90.0% as determined by SDS-PAGE.BP001767-C588: Recombinant Human C-Type Lectin Domain Family 4 Member E (CLEC4E) Protein
Source: HEK 293-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).BP002931-PRO-1131: Recombinant Human NmrA-like family domain containing protein 1 (NMRAL1) Protein
Source: E. coli-derived.
Purity: >95.0% as determined by SDS-PAGE.BP000040-0011: Recombinant Mouse C-C Motif Chemokine 1 (CCL1) Protein
Source: CHO cell cell-derived.
Purity: > 92% by SDS-PAGE.
Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
Fusion protein: Human Fc fused to the C-terminus of mouse CCL1.BP000144-CYT-152: Recombinant Rat Interleukin-1 Receptor Antagonist Protein (IL-1RA)
Source: E. coli-derived.
Fully biologically active when compared to standard. Measured by its ability to inhibit IL1-dependent proliferation in D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 30-150 ng/ml (corresponding to a specific activity of 6,667-33,334 units/mg ) in the presence of 50 pg/ml of rrIL1.
Purity: > 95.0% by SDS-PAGE.