BP004279: Biotinylated Human IL-18 Protein with No Tag
Source: The recombinant Biotinylated Human IL-18 Protein, (amino acids Tyr37-Asp193) fused with No Tag, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-18BP, hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-18, No tag with the EC50 of 5.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005065: Biotinylated Human IL-18BP Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-18BP Protein, (amino acids Thr31-Gly194) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 0.90 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.EK000020-EK0864: Human IL-18 ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 1 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human IL-18 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.BP005730: Human IL-18 Protein with No Tag
Source: The recombinant Human IL-18 Protein, (amino acids Tyr37-Asp193) fused with No Tag, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-18 at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-18BP, hFc tag with the EC50 of 16.9 ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005004: Human IL-18BP Protein with the C-His Tag
Source: The recombinant Human IL-18BP Protein, (amino acids Thr31-Gly194) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004887: Human IL-18BP Protein with the C-hFc Tag
Source: The recombinant Human IL-18BP Protein, (amino acids Thr31-Gly194) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IL-18 protein with the C-hFc tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-18BP protein with the C-hFc tag with the EC50 of 16.9 ng/ml determined by ELISA (QC Test). The affinity constant of 0.04 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000142-CYT-269: Recombinant Human IL-18
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of the IFN-g by human PBMC co-stimulated with IL-12 was found to be 5 ug/ml corresponding to a Specific Activity of 200,000 IU/mg.
Purity > 98%, by SDS-PAGE and RP-HPLC.BP000142-CYT-376: Recombinant Rat IL-18
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of IFN-gamma by murine lymph node cells (400,000 cells/well) co-stimulated with sub optimal dose of ConA (0.75 ug/ml) is 12 ug/ml corresponding to a specific activity of 84,000 U/mg. The medium was RPMI containing 10% FCS and 50 2-ME.
Purity > 95%, by SDS-PAGE and RP-HPLC.BP000142-CYT-634: Recombinant Mouse IL-18
Source: E. coli-derived.
Purity > 90%, by SDS-PAGE.BP000142-CYT-663: Recombinant Human IL-18, His Tag
Source: E. coli-derived.
Purity > 95%, by SDS-PAGE.BP004873: Mouse IL-18BP Protein with the C-His Tag
Source: The recombinant Mouse IL-18BP Protein, (amino acids Thr29-Ala193) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse IL-18BP protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated IL-18 protein with the C-His tag with the EC50 of 23.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.86 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004866: Mouse IL-18BP Protein with the C-hFc Tag
Source: The recombinant Mouse IL-18BP Protein, (amino acids Thr29-Ala193) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity:Immobilized recombinant Mouse IL-18BP protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-18 protein with the C-hFc tag with the EC50 of 8.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.