BP005643: Biotinylated Human CTLA-4/CD152 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human CTLA-4/CD152 Protein, (amino acids Lys36-Asp161) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human CTLA-4 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human B7-1 protein with the C-hFc tag with the EC50 of 2.4 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005158: Cynomolgus CTLA-4/CD152 Protein with the C-His Tag
Source: The recombinant Cynomolgus CTLA-4/CD152 Protein, (amino acids Ala37-Ser160) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus CTLA-4 protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CTLA-4 Antibody protein with the C-hFc tag with the EC50 of 11.2 ng/ml determined by ELISA (QC Test). The affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005332: Human CTLA-4/CD152 Protein with the C-His Tag
Source: The recombinant Human CTLA-4/CD152 Protein, (amino acids Lys36-Asp161) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CTLA-4 protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-CTLA-4 Antibody protein with the C-hFc tag with the EC50 of 12.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005051: Human CTLA-4/CD152 Protein with the C-hFc Tag
Source: The recombinant Human CTLA-4/CD152 Protein, (amino acids Lys36-Asp161) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human B7-2 protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CTLA-4 protein with the C-hFc tag with the EC50 of 0.12 ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.