PA000274.m1: Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

The in vivo grade recombinant anti-DYKDDDDK-Tag monoclonal antibody (clone L1.1), mouse IgG1 kappa, is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions. 
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Reactivity: Human, Mouse, Rat, Others.
Clone: L1.1.
Isotype: mouse IgG1 kappa.

PA000274.h1: In vivo Grade Recombinant Anti-DYKDDDDK-Tag Human IgG1 Monoclonal Antibody (clone L1.1)

The in vivo grade recombinant anti-DYKDDDDK-Tag monoclonal antibody (clone L1.1), human IgG1 kappa, is a chimeric mouse/human monoclonal antibody produced in mammalian cells. It consists of mouse variable regions and human IgG1 kappa constant regions. 
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Reactivity: Human, Mouse, Rat, Others.
Clone: L1.1.
Isotype: human IgG1 kappa.

20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.

PA007126: In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody

The in vivo grade recombinant mouse IgG1 isotype control antibody was produced in CHO cells.
Applications: an isotype-matched negative control for mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007135: In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody

The in vivo grade recombinant mouse IgG1-D265A isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for mouse IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007144: In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody

The in vivo grade recombinant rat IgG2b isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for rat IgG2b kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007143: In Vivo Grade Recombinant Rat IgG2a Isotype Control Antibody

The in vivo grade recombinant rat IgG2a isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for rat IgG2a kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007142: In Vivo Grade Recombinant Rat IgG1 Isotype Control Antibody (rtIgG1 Isotype Control)

The in vivo grade recombinant rat IgG1 isotype control antibody (rtIgG1 isotype control) was produced in mammalian cells.
Applications: an isotype-matched negative control for rat IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007147: In Vivo Grade Recombinant Armenian Hamster IgG2 Isotype Control Antibody

The in vivo grade recombinant Armenian hamster IgG2 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG2 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007146: In Vivo Grade Recombinant Armenian Hamster IgG1 Isotype Control Antibody

The in vivo grade recombinant hamster IgG1 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007148: In Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody

The in vivo grade recombinant hamster IgG1 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

C043P: Daclizumab Biosimilar, CD25 Monoclonal Antibody

Recombinant Humanized IgG1 Monoclonal Antibody.
Specificity/Sensitivity: The in vivo grade daclizumab biosimilar specifically binds to the human interleukin-2 receptor (anti-Tac or anti-CD25).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by daclizumab.

Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.

A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.

Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.