BP000020-CYT-657: Recombinant Bovine Fibroblast Growth Factor 21 (FGF-21) Protein
Source: E. coli-derived.
Purity: > 98.0% by Gel Filtratio and SDS-PAGEBP002204-PRO-1021: Recombinant Human Tumor Suppressing Subtransferable Candidate 4 (TSSC4) Protein
Source: E. coli-derived.
Purity: > 90% as determined by SDS-PAGE.BP002517-PRO-823: Recombinant Human Hydroxysteroid (17-beta) Dehydrogenase 10 (HSD17B10) Enzyme
Source: E. coli-derived.
Purity: > 95% as determined by SDS-PAGE.BP002892-PRO-885: Recombinant Human Developmentally Regulated GTP Binding Protein 1 (DRG1)
Source: E. coli-derived.
Purity: > 85.0% as determined by SDS-PAGE.Single B cell antibody cloning and sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by Sanger DNA sequencing or next generation sequencing (NGS) has been successfully applied to single B cell antibody technologies.
Syd Labs generates scFv phage libraries from immunized or naive donors from several species. Additionally, various mutagenesis strategies can be used to design scFv phage libraries for affinity maturation of a given antibody or other optimization purposes. Our scFv phage library is generated with a goal of up to 1010 clones and quality controlled by sequencing 96 clones at random.
Syd Labs screens scFv phage libraries using several different formats for applications including de novo antibody discovery and affinity maturation of existing antibodies.
The antibody variable regions of both heavy chain (VH) and light chain (VL) are analyzed for liabilities including, but not limited to N-linked glycosylation, deamidation, isomerization, pyroglutamate formation, and oxidation.
Fast turnaround time: 1 week per antibody, but may require more time depending on the complexity of analysis.N-terminal Edman sequencing of proteins or peptides can be used to determine the N-terminal amino acid residues of purified protein samples in addition to cleavage sites, and to identify an unknown protein or confirm the identity of a known protein, or to design oligonucleotide probes.
High sensitivity: even with picomoles of protein.
Various formats: in solution, gels or on PVDF blots.
Fast turnaround time: 1-2 weeks per sample, but may require more time depending on the complexity of analysis.Kinetic analysis is used to determine the affinity of an antibody to its soluble antigen using surface plasmon resonance (SPR) or biolayer interferometry (BLI), which can measure kon, koff, and kD. Association and dissociation are measured on the Biacore T100 or the ForteBio Octet QKe using various concentrations of the antigen and the kD is calculated using an appropriate curve-fitting model.
Fast turnaround time: 2-3 days per antibody-antigen pair, but may require more time depending on the complexity of analysis.