BP004780: Human IGFBP-3 Protein with the C-His Tag
Source: The recombinant Human IGFBP-3 Protein, (amino acids Gly28-Lys291) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IGFBP-3 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human IGF-II protein with the C-hFc tag with the EC50 of 0.19 ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
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