BP003857: Human TNFRSF11B Protein with the C-His Tag
Source: The recombinant Human TNFRSF11B Protein, (amino acids Glu22-Leu401) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human RANKL protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11B protein with the C-His tag with the EC50 of 16.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
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