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Phone: 1-617-401-8149
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BP003963: Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, (amino acids Phe31-Val992(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer protein with the His-Avi tag at the C-terminus has a predicted MW of 114 kDa (ITGAV) & 81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 100-160 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Biotinylated Human ITGAV&ITGB3 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on streptavidin (5 ug/ml) precoated plate. Dose response curve for the recombinant Anti-alpha V beta3 Antibody protein with the C-hFc tag with the EC50 of 4.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs also provides the following alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein:
Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein
Biotinylated Mouse Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein
Cynomolgus Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein
Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein
Mouse Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein
Syd Labs provides the following ITGAV Antibody:
ITGAV Antibody
Syd Labs also provides the following ITGAV&ITGB1/ITGAV&ITGB8 Protein:
Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein
Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein
Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein
Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein
Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein
Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein
Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein
Mouse Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Rhesus macaque Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
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