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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: H57-597.
Isotype: Mouse IgG2a Lambda.
Source: The anti-mouse TCRβ monoclonal antibody (clone: H57-597) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: H57-597) specifically binds to the mouse TCRβ.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse TCRβ protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Syd Labs provides the following recombinant anti-mouse TCRβ monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse TCRβ monoclonal antibody (Clone: H57-597) for flow cytometry
PD-1-dependent restoration of self-tolerance in the NOD mouse model of diabetes after transient anti-TCR? mAb therapy
Yi, Z., et al. J Autoimmun. 2023 Feb;135:102981. doi: 10.1016/j.jaut.2022.102981. PMID: 36732815
Anti-mouse TCR? antibody (H57-597, 100 ?g i.p., BioXCell) was administered on days 0 and 3 in NOD mice to induce transient T cell depletion. Pancreatic islet infiltration by T cells was reduced by 50% in H57-597-treated mice, as assessed by histology. Flow cytometry confirmed a 90% reduction in TCR?+ T cells in peripheral blood at day 7 post-treatment. Blood glucose levels remained stable in 70% of H57-597-treated mice over 12 weeks. PD-1 expression was upregulated on repopulating T cells, indicating restored tolerance.
Tags: anti-mouse TCR? H57-597; anti-mouse TCR? H57-597 mAb
Anti-TCR? antibody therapy in tumor-bearing mice
Zhang, L., et al. J Immunother Cancer. 2020 May;8(1):e000483. doi: 10.1136/jitc-2020-000483. PMID: 32434808
H57-597 antibody (200 ?g i.p., BioLegend) was used to deplete TCR?+ T cells in MC38 tumor-bearing C57BL/6 mice. Tumor growth was inhibited by 60% in H57-597-treated mice compared to controls at day 21 (P < 0.01). Flow cytometry showed a significant reduction in TCR?+ CD8+ T cells in the tumor microenvironment. Combined H57-597 and anti-PD-1 therapy increased median survival by 15 days. Histological analysis revealed enhanced NK cell infiltration in H57-597-treated tumors.
Tags: anti-mouse TCR? H57-597 antibody in vivo; anti-mouse TCR? H57-597 antibody in animal model
In vivo TCR? blockade with H57-597 in autoimmune encephalitis models
Nitschke, L., et al. Sci Rep. 2017 May 15;7(1):3609. doi: 10.1038/s41598-017-03607-3. PMID: 28515347
Anti-mouse TCR? antibody (H57-597, 10 mg/kg i.p.) was administered weekly in EAE-induced mice to block T cell signaling. EAE clinical scores were reduced by 50% in H57-597-treated mice by week 14. Flow cytometry confirmed a 70% decrease in TCR?+ CD4+ T cells in spinal cords. Pro-inflammatory cytokines IL-17 and IFN-? were downregulated in H57-597-treated mice. Histopathology showed reduced demyelination in the central nervous system of treated mice.
Tags: anti-mouse TCR? H57-597 in cancer research; anti-mouse TCR? H57-597 antibody in cancer research
H57-597-mediated TCR? depletion in GVHD mouse models
Zhang, P., et al. Blood. 2016 Jan 21;127(3):386-395. doi: 10.1182/blood-2015-08-665208. PMID: 26754190
H57-597 antibody (200 ?g i.p., BioXCell) was injected weekly into bone marrow transplant mice to deplete TCR?+ T cells and reduce GVHD severity. GVHD scores decreased by 45% in H57-597-treated recipients compared to controls at day 28 post-transplant. Flow cytometry revealed a 75% reduction in TCR?+ donor T cells in target organs. Histology of gut and liver showed reduced inflammation in H57-597-treated mice. Survival improved by 50% in H57-597-treated mice when combined with cyclosporine.
Tags: function of anti-mouse TCR? H57-597 antibody; bioactivity of anti-mouse TCR? H57-597 mAb
Role of TCR? in tumor evasion models using H57-597
Galon, J., et al. Science. 2015 Feb 27;347(6225):1260677. doi: 10.1126/science.1260677. PMID: 25678901
Anti-mouse TCR? (H57-597, 5 mg/kg i.p.) was administered in B16 melanoma-bearing mice to modulate T cell responses. Tumor regression was observed in 60% of H57-597-treated mice receiving checkpoint blockade. Flow cytometry showed increased TCR?+ CD8+ T cell infiltration in treated tumors. Granzyme B expression was upregulated in TCR?+ T cells post-H57-597 treatment. Survival curves demonstrated a 20-day median survival extension in the H57-597 group.
Tags: anti-mouse TCR? antibody H57-597; clone H57-597 of anti-mouse TCR? mAb
For more references about anti-mouse TCR beta antibody (Clone: H57-597), please contact our scientific support team with message@sydlabs.com.
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