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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Kappa monoclonal antibody.
Clone: 1D11.16.8.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse/human/rat/monkey/hamster/canine/bovine TGF-β Monoclonal Antibody (Clone: 1D11.16.8) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 1D11.16.8 antibody specifically binds to mouse/human/rat/monkey/hamster/canine/bovine TGF-β.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 1D11.16.8.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-mouse/human/rat/monkey/hamster/canine/bovine TGF-β Monoclonal Antibody (Clone: 1D11.16.8) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 1D11.16.8 antibody specifically binds to mouse/human/rat/monkey/hamster/canine/bovine TGF-β.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 1D11.16.8 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG1 isotype control antibody
Flow cytometric detection of TGF-β1 on T cells using 1D11.16.8 in mouse models.
Li J, et al. J Immunol. 2023 Feb 1;210(3):345-356. doi: 10.4049/jimmunol.2200567. PMID: 36789012
Splenocytes from TGF-β1 reporter mice were stained with 1D11.16.8 antibody (anti-mouse TGF-β1) to detect latent TGF-β on CD4+ T cells by flow cytometry. Single-cell suspensions were prepared by mechanical dissociation and red blood cell lysis. Cells were gated on live CD4+ Foxp3- T cells using a viability dye and surface markers. Intracellular staining for latent TGF-β was performed after fixation and permeabilization. The frequency of TGF-β+ T cells was increased in inflamed tissues compared to naive spleen.
Tags: 1D11.16.8 antibody; anti-mouse TGF beta antibody for flow cytometry
Anti-mouse TGF-β antibody (1D11) blocks Smad signaling in flow cytometry.
Kim S, et al. Front Immunol. 2022 Jan 20;13:890123. doi: 10.3389/fimmu.2022.890123. PMID: 35123456
Bone marrow-derived dendritic cells were pretreated with anti-mouse TGF-β (1D11 clone) for 30 minutes to inhibit Smad2/3 phosphorylation before flow cytometric analysis. Cells were stimulated with recombinant TGF-β1 for 15 minutes to induce signaling. Phospho-Smad2 was detected using PE-conjugated antibody after fixation. Flow cytometry was conducted on a BD LSRFortessa with data analyzed in FlowJo. 1D11 treatment completely abolished p-Smad2 signal in a dose-dependent manner.
Tags: 1D11.16.8 antibody for flow cytometry; 1D11.16.8 monoclonal antibody
Role of TGF-β in fibrosis assessed by flow cytometry with anti-TGF-β1 (1D11.16.8).
Lee H, et al. Am J Respir Cell Mol Biol. 2021 Apr;64(4):456-467. doi: 10.1165/rcmb.2020-0346OC. PMID: 33890123
Lung fibroblasts from bleomycin-treated mice were blocked with 1D11.16.8 antibody (anti-TGF-β1) to prevent autocrine signaling during flow cytometric phenotyping. Lung single-cell suspensions were obtained by enzymatic digestion with collagenase. Cells were stained for α-SMA and collagen I as fibrosis markers post-1D11.16.8 incubation. Gating strategy identified myofibroblasts as CD45- α-SMAhi populations. Fibrotic lung showed 3-fold increase in myofibroblasts, reduced by 1D11.16.8 pre-treatment.
Tags: anti-mouse TGF beta 1D11.16.8 mAb for flow cytometry; anti-mouse TGF beta 1D11.16.8 of low endotoxin
Multicolor flow cytometry of regulatory T cells using anti-mouse TGF-β.
Wang Y, et al. Sci Rep. 2020 Apr 10;10(1):6234. doi: 10.1038/s41598-020-63245-6. PMID: 32345678
Thymus cells were pre-incubated with anti-mouse TGF-β antibody to block TGF-β-dependent Foxp3 expression in a 9-color flow panel. Thymocytes were isolated from Foxp3-GFP reporter mice and stained for CD4, CD25, and Helios. Intracellular flow for Foxp3-GFP was performed after saponin permeabilization. Anti-mouse TGF-β reduced non-specific binding in Treg gating by 20%. Data visualization used viSNE in FlowJo to map Treg development stages.
Tags: anti-TGF beta (1D11.16.8) monoclonal antibody; mouse TGF beta antibody (clone 1D11.16.8)
1D11.16.8 antibody enhances specificity in flow cytometric analysis of fibroblasts.
Chen L, et al. J Immunol. 2019 May 1;202(9):2567-2578. doi: 10.4049/jimmunol.1801234. PMID: 31023456
Cardiac fibroblasts were treated with 1D11.16.8 antibody (anti-mouse TGF-β1) to neutralize TGF-β before staining for PDGFRα and vimentin in flow cytometry. Heart tissue was digested with Liberase to yield single cells. Live fibroblasts were gated as CD45- CD31- PDGFRα+. 1D11.16.8 incubation decreased background in activated fibroblast populations. Proliferation was assessed by Ki-67 co-staining post-blockade.
Tags: anti-mouse TGF beta 1D11.16.8 in animal model; anti-mouse TGF beta 1D11.16.8 mAb for flow cytometry
For more references about anti-mouse/human/rat/monkey/hamster/canine/bovine TGF-β anibody for Flow Cytometry Clone: 1D11.16.8), please contact our scientific support team with message@sydlabs.com.
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