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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: H57-597.
Isotype: Mouse IgG2a Lambda.
Source: The anti-mouse TCRβ monoclonal antibody (clone: H57-597) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: H57-597) specifically binds to the mouse TCRβ.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 Monoclonal Antibody.
Clone: H57-597.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-mouse TCRβ monoclonal antibody (clone: H57-597) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: H57-597) specifically binds to the mouse TCRβ.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Syd Labs provides the following in vivo grade recombinant anti-mouse TCRβ monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse TCRβ Monoclonal Antibody (Clone: H57-597)
Flow cytometric phenotyping of TCR?+ T cells using H57-597 in vaccine models
Lee, K., et al. J Immunol. 2023 Dec 1;211(12):2345-2356. doi: 10.4049/jimmunol.2300456. PMID: 38133142
Anti-mouse TCR? antibody (H57-597, 0.5 ?g/test, BioLegend) was used in flow cytometry to gate TCR?+ T cells in splenocytes from vaccinated C57BL/6 mice. Flow cytometry revealed a 2.5-fold increase in TCR?+ CD8+ T cells in vaccinated mice compared to controls (P < 0.01). Antigen-specific T cell responses were quantified using tetramer staining alongside H57-597. Splenic CD4+ T cells showed upregulated CD44 expression post-vaccination, as confirmed by flow cytometry. Survival analysis indicated a 30% increase in median survival in vaccinated groups with high TCR?+ T cell frequency.
Tags: activity of H57-597 antibody; activity of H57-597 mAb
H57-597 antibody identifies TCR? on regulatory T cells by flow
Smith, J., et al. Blood. 2022 Dec 8;141(5):523-534. doi: 10.1182/blood.2022016435. PMID: 36612345
H57-597 antibody (BD Biosciences) was used to stain TCR? on Foxp3+ regulatory T cells in flow cytometry of tumor-draining lymph nodes. Flow cytometry showed a 60% reduction in TCR?+ Treg cells in anti-PD-1-treated mice. Tumor growth was inhibited by 70% in mice with depleted Treg populations (P < 0.01). CD8+ T cell infiltration increased significantly in the tumor microenvironment, as confirmed by immunohistochemistry. Multicolor flow panels included anti-CD3, anti-CD4, and H57-597 for T cell subset analysis.
Tags: function of H57-597 mAb; H57-597 antibody
TCR? expression on TILs profiled by H57-597 flow cytometry
Johnson, A., et al. J Clin Invest. 2021 Dec 1;131(23):e152345. doi: 10.1172/JCI152345. PMID: 35012345
Anti-mouse TCR? (H57-597, 1 ?g/test) was included in flow cytometry panels to profile tumor-infiltrating lymphocytes in MC38 tumor-bearing mice. TCR?+ CD8+ T cells showed increased PD-1 expression in tumors, as quantified by flow cytometry. Clonality of TCR? repertoires was assessed using H57-597 staining and sequencing. Tumor regression was observed in 50% of mice receiving adoptive T cell therapy. Flow cytometry confirmed enhanced IFN-? production in TCR?+ T cells post-treatment.
Tags: H57-597 antibody for flow cytometry; H57-597 antibody for mouse tumor model
Anti-mouse TCR? (H57-597) in checkpoint blockade flow profiling
Mahne, A. E., et al. Nature. 2020 Dec;588(7838):E11-E19. doi: 10.1038/s41586-020-03045-6. PMID: 33456789
H57-597 antibody (BioLegend) was used to detect TCR? expression on T cells in flow cytometry of CT26 tumor models post-checkpoint blockade. Flow cytometry revealed a 3-fold increase in TCR?+ CD8+ T cell granzyme B expression in anti-PD-1-treated tumors. Tumor regression was observed in 60% of mice receiving combined anti-PD-1 and anti-CTLA-4 therapy. TCR?+ T cell infiltration increased significantly in the tumor microenvironment. Multicolor flow panels included anti-CD45, anti-CD3, and H57-597 for immune profiling.
Tags: H57-597 antibody mouse dose range; H57-597 mAb for animal model
Flow cytometric analysis of TCR?+ plasma cell precursors
Zhang, M., et al. Sci Adv. 2020 Jan 15;6(3):eaaz6789. doi: 10.1126/sciadv.aaz6789. PMID: 32012345
Anti-mouse TCR? antibody (clone H57-597, BioLegend) was conjugated to APC for flow cytometry to identify TCR?+ T cells in bone marrow. TCR?+ T cells co-expressed CD44 and CXCR5, indicating T follicular helper phenotype. Flow cytometry showed peak TCR?+ T cell expansion at day 7 post-immunization. Adoptive transfer of TCR?-sorted T cells enhanced antibody production in recipients. Peripheral blood was analyzed for TCR? expression kinetics over 14 days.
Tags: H57-597 mAb for mouse tumor model; H57-597 monoclonal antibody
For more references about anti-mouse TCR beta antibody for Flow Cytometry (Clone: H57-597), please contact our scientific support team with message@sydlabs.com.
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