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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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In vivo grade recombinant DYKDDDDK-Tag monoclonal antibodies with various isotypes; alternative to the anti-FLAG M2 antibody; for in vitro and in vivo studies including protein purification; low prices for bulk order. FLAG is a registered trade mark of Sigma Aldrich, and used here for informational purposes only.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The recombinant anti-DYKDDDDK-Tag mouse IgG1 monoclonal antibody (clone M2.1) is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions.
Clone: M2.1.
Isotype: mouse IgG1 kappa.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody binds to the same epitope as Sigma 'Anti-FLAG' M2 antibody.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein G.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
The in vivo grade recombinant anti-DYKDDDDK-Tag human IgG1 monoclonal antibody (clone M2.1) is a chimeric mouse/human monoclonal antibody produced in mammalian cells. It consists of mouse variable regions and human IgG1 kappa constant regions.
Clone: M2.1.
Isotype: human IgG1 kappa.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The recombinant anti-DYKDDDDK-Tag monoclonal antibody (clone M2.1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
Other DYKDDDDK Antibodies:
DYKDDDDK-tag Monoclonal Antibody
DYKDDDDK-tag Monoclonal Antibody (Magnetic Microbead-Conjugated)
DYKDDDDK-tag Monoclonal Antibody (Agarose-Conjugated)
DYKDDDDK Peptide:
DYKDDDDK Peptide
Other Tag Antibodies:
His-tag Monoclonal Antibody
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
HA-tag Monoclonal Antibody
Myc-tag Monoclonal Antibody
V5-tag Monoclonal Antibody
Loading Control Antibodies:
GAPDH Monoclonal Antibody
beta-Actin Monoclonal Antibody
beta-Tubulin Monoclonal Antibody
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