Anti-Human IgG Monoclonal Antibodies

Anti-human IgG specific antibodies are useful in preclinical animal studies, such as pharmacokinetic (PK), especially if they do not react with non-human primate and murine IgG samples. There are both polyclonal and monoclonal antibodies for such applications. 

Anti-human IgG specific polyclonal antibodies: IgG (Fc)-targeting and IgG (Intact / H+L)-targeting polyclonal secondary antibodies are affinity-purified and monkey-absorbed to minimize species cross-reactivity with monkey serum proteins.

Anti-human IgG specific monoclonal antibodies: They are screened not to recognize IgG isotypes from the non-human primate species such as rhesus and cynomolgus macaques. Ideally, they do not recognize mouse or rat IgG isotypes.

M252Y/S254T/T256E (YTE) mutations in the Fc region of human IgG antibodies can prolong the half-life of therapeutic antibodies, optimize the pharmacokinetic characteristics, and reduce the frequency of administration. Our recombinant rabbit anti-human IgG Fc (YTE mutation) specific monoclonal antibodies effectively capture and stably bind to human IgG Fc segment YTE mutants. 

PA006000: Rabbit Anti-Human IgG Monoclonal Antibody

Rabbit Monoclonal Antibody.
Clone: 32RA04
Isotype: Rabbit IgG1 kappa.
Source: The recombinant rabbit anti-human IgG monoclonal antibody (clone: 32RA04) was produced in mammalian cells.
Reactivity: Human.
Purification: The recombinant rabbit anti-human IgG monoclonal antibody was affinity-purified from cell culture supernatant by affinity-chromatography using protein A.
Specificity/Sensitivity: The recombinant rabbit anti-human IgG monoclonal antibody (clone: 32RA04) specifically recognizes human immunoglobulin isotypes (IgG1, IgG2, IgG3, IgG4), total IgG and IgG-Fab but NOT Cynomolgus macaque total IgG, Rhesus macaque total IgG, Marmoset total IgG, Mouse total IgG, and Rat total IgG.
Applications: ELISA, Western Blot, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006001: Rabbit Anti-Human IgG Monoclonal Antibody

Rabbit Monoclonal Antibody.
Clone: 28RA05
Isotype: Rabbit IgG1 kappa.
Source: The recombinant rabbit anti-human IgG monoclonal antibody (clone: 28RA05) was produced in mammalian cells.
Reactivity: Human.
Purification: The recombinant rabbit anti-human IgG monoclonal antibody was affinity-purified from cell culture supernatant by affinity-chromatography using protein A.
Specificity/Sensitivity: The recombinant rabbit anti-human IgG monoclonal antibody (clone: 28RA05) specifically recognizes human immunoglobulin isotypes (IgG1, IgG2, IgG3, IgG4), total IgG but NOT Cynomolgus macaque total IgG, Rhesus macaque total IgG, Marmoset total IgG, Mouse total IgG, and Rat total IgG.
Applications: ELISA, Western Blot, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006002: Rabbit Anti-Human IgG Fc (YTE Mutation) Monoclonal Antibody

Rabbit Monoclonal Antibody.
Clone: 21RA09
Isotype: Rabbit IgG1 kappa.
Source: The recombinant rabbit anti-human IgG Fc (YTE mutation, M252Y/S254T/T256E) monoclonal antibody (clone: 21RA09) was produced in mammalian cells.
Reactivity: Human.
Purification: The recombinant rabbit anti-human IgG Fc (YTE mutation) monoclonal antibody was affinity-purified from cell culture supernatant by affinity-chromatography using protein A.
Specificity/Sensitivity: The recombinant rabbit anti-human IgG Fc (YTE mutation) monoclonal antibody (clone: 21RA09) specifically recognizes human IgG Fc with the YTE mutation.
Applications: ELISA, Western Blot, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006003: Rabbit Anti-Human IgG Fc (YTE Mutation) Monoclonal Antibody

Rabbit Monoclonal Antibody.
Clone: 6RA10
Isotype: Rabbit IgG1 kappa.
Source: The recombinant rabbit anti-human IgG Fc (YTE mutation, M252Y/S254T/T256E) monoclonal antibody (clone: 6RA10) was produced in mammalian cells.
Reactivity: Human.
Purification: The recombinant rabbit anti-human IgG Fc (YTE mutation) monoclonal antibody was affinity-purified from cell culture supernatant by affinity-chromatography using protein A.
Specificity/Sensitivity: The recombinant rabbit anti-human IgG Fc (YTE mutation) monoclonal antibody (clone: 6RA10) specifically recognizes human IgG Fc with the YTE mutation.
Applications: ELISA, Western Blot, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA006004: Rabbit Anti-Human IgG1 Fc (DLE Mutation) Monoclonal Antibody

Rabbit Monoclonal Antibody.
Clone: 4RA11
Isotype: Rabbit IgG1 kappa.
Source: The recombinant rabbit anti-human IgG1 Fc (DLE mutation, S239D/A330L/I332E) monoclonal antibody (clone: 4RA11) was produced in mammalian cells.
Reactivity: Human.
Purification: The recombinant rabbit anti-human IgG1 Fc (DLE mutation) monoclonal antibody was affinity-purified from cell culture supernatant by affinity-chromatography using protein A.
Specificity/Sensitivity: The recombinant rabbit anti-human IgG Fc (DLE mutation) monoclonal antibody (clone: 4RA11) specifically recognizes human IgG1 Fc with the DLE mutation.
Applications: ELISA, Western Blot, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Other Secondary Antibodies:
Recombinant Anti-rat Kappa Light Chain Monoclonal Antibody (Clone: MAR18.5)