BP005323: Human ANGPT2/Angiopoietin-2 Protein with the N-His-Avi Tag
Source: The recombinant Human ANGPT2/Angiopoietin-2 Protein, (amino acids Lys275-Phe496) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human ANGPT2 protein with the N-His-Avi tag at 2 ug/ml (100 ul/well). Dose response curve for the recombinant Anti-ANGPT2 Antibody protein with the N-hFc tag with the EC50 of 9.3 ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005693: Biotinylated Human ANGPT2/Angiopoietin-2 Protein with the N-His-Avi Tag
Source: The recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein, (amino acids Lys275-Phe496) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human ANGPT2 protein with the N-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Anti-ANGPT2 Antibody protein with the N-hFc tag with the EC50 of 3.8 ng/ml determined by ELISA (QC Test). The affinity constant of 2.93 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.