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The best anti-HA-tag antibody; for various in vitro and in vivo studies including protein purification. FLAG is a registered trade mark of Sigma Aldrich, and used here for informational purposes only.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The recombinant anti-DYKDDDDK / HA-Tag bispecific antibody (mouse IgG2a kappa) was produced in mammalian cells.
Clone: M2.3 / 12CA5.
Isotype: mouse IgG2a, kappa.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins, the 9-amino acid sequence YPYDVPDYA, derived from influenza virus hemagglutinin (HA) on HA tagged proteins and HA tag expression cells.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Reactivity: Human, Mouse, Rat, Others.
Purification: Protein A.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The recombinant anti-DYKDDDDK / HA-Tag bispecific antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
Other DYKDDDDK Antibodies:
DYKDDDDK-tag Monoclonal Antibody (Clone M2.1)
DYKDDDDK-tag Monoclonal Antibody
DYKDDDDK-tag Monoclonal Antibody (Magnetic Microbead-Conjugated)
DYKDDDDK-tag Monoclonal Antibody (Agarose-Conjugated)
DYKDDDDK Peptide:
DYKDDDDK Peptide
Other Tag Antibodies:
HA-Tag Monoclonal Antibody (Clone 12CA5)
His-Tag Monoclonal Antibody (Clone 3D5)
Myc-Tag Monoclonal Antibody (Clone 9E10)
His-tag Monoclonal Antibody (Clone HIS.H8)
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
His-Tag Monoclonal Antibody (Clone 26D11)
V5-tag Monoclonal Antibody (Clone V5.E3)
Recombinant anti-DYKDDDDK-tag Bispecific Antibodies:
Recombinant Anti-DYKDDDDK / c-Myc-Tag Bispecific Antibody
Recombinant Anti-DYKDDDDK / HA-Tag Bispecific Antibody
Recombinant Anti-DYKDDDDK / His-Tag Bispecific Antibody
Questions and Answers about recombinant anti-DYKDDDDK / HA-Tag bispecific antibody (Clone M2.3 / 12CA5):
Question: Why is the anti-DYKDDDDK / HA-tag bispecific antibody the best anti-HA-tag antibody?
Answer: If there are both DYKDDDDK and HA-tags in the same proteins of interest, the M2.3 antibody in the M2.3 / 12CA5 bispecific antibody help signicantly improving the affinity of the 12CA5 antibody to the HA-tagged proteins.
Question: Can the DYKDDDDK-tag and HA-tag be in different proteins?
Answer: Yes, the DYKDDDDK-tag and HA-tag can be in different proteins of interest even if the proteins are in different protein complexes or on different cells. The anti-DYKDDDDK / HA-tag bispecific antibody may bring two proteins, protein complexes and even cells together.
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