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Recombinant SARS-CoV-2 Spike S1 Protein

2019-nCoV S Protein S1 Subunit, COVID-19 Virus Spike Protein S1 Subunit

Catalog No. Product Name Size List Price (US$) Quantity
BP003054 Recombinant SARS-CoV-2 Spike S1-His-Avi Protein 100 ug 380.00
BP003054 Recombinant SARS-CoV-2 Spike S1-His-Avi Protein 1 mg 2300.00
BP003071 Recombinant SARS Spike S1-His-Avi Protein 100 ug 380.00
BP003071 Recombinant SARS Spike S1-His-Avi Protein 1 mg 2300.00
Description

BP003054: Recombinant SARS-CoV-2 Spike S1-His-Avi Protein (Spike Protein S1 Subunit)

Source: recombinant SARS-CoV-2 spike protein S1 subunit (Spike S1 protein, Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 77.9 kDa. Due to glycosylation, the recombinant SARS-CoV-2 spike S1 protein migrates to 110-120 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Activity: Immobilized recombinant SARS-CoV-2 Spike S1-His-Avi protein at 0.5 ug/ml (100 ul/well). Dose response curve for the biotinylated recombinant human ACE2-His fusion protein with the EC50 of 0.2 ug/ml determined by ELISA.
Formulation: The recombinant SARS-CoV-2 Spike S1-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.

Shipping: The recombinant SARS-CoV-2 Spike S1-His-Avi protein is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Questions and Answers:

Question: Is Syd Labs's recombinant SARS-CoV-2 spike S1 protein trimer or monomer?
Answer: Different from the SARS-CoV-2 spike protein in the virus, the recombinant SARS-CoV-2 spike S1 subunit is a monomer.

Question: Does Syd Labs have SARS-CoV-2 spike protein S1 subunit-based rapid test kit or ELISA kit?
Answer: Yes, please check our SARS-CoV-2 IgG ELISA kit (S protein).

Question: What is the advantage of SARS-CoV-2 spike S1 protein-based immunoassay kits to nucleocapsid-based kits?
Answer: SARS-CoV-2 S1 subunit-based immunoassay kits have better specificity though lower sensitivity.

Question: Is SARS-CoV-2 spike S1 protein or its RBD a better immunogen for antibody development?
Answer: SARS-CoV-2 S1 subunit is better since it is bigger with more epitopes possibly better for antibody development, especially for neutralizing antibodies. Published and unpublished results indicate that some RBD antibodies binds to RBD but does not block the interaction between RBD and the receptor ACE2.

SARS-CoV-2 (2019 nCoV or COVID-19 virus) proteins:

Biotinylated Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-Fc-Avi
Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein
Recombinant SARS-CoV-2 Spike S-Fc-Avi Protein
Biotinylated Recombinant SARS Spike RBD-His-Avi
Recombinant SARS Spike RBD-His-Avi
Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi
Biotinylated Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-Fc-Avi Protein

SARS-CoV-2 (2019 nCoV or COVID-19 virus) antibodies:

SARS-CoV-2 Spike RBD Monoclonal Antibody

SARS-CoV-2 (2019 nCoV or COVID-19 virus) monoclonal antibody development services:

SARS-CoV-2-specific human neutralizing monoclonal antibody development service
SARS-CoV-2 Spike S1 rabbit monoclonal antibody development service
SARS-CoV-2 Nucleocapsid rabbit monoclonal antibody development service

SARS-CoV-2 (2019 nCoV or COVID-19 virus) detection kits:

SARS-CoV-2 IgG ELISA kit (S protein)
SARS-CoV-2 IgG ELISA kit (N protein)

To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services

Background

There are two subunits, S1 and S2, in the SARS-CoV-2 spike (S) glycoprotein which plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of virus entry inhibitors, neutralizing antibodies, and vaccines. The S1 subunit, the N-terminal 14–685 amino acids of S protein, contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM). The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit.

The His-tagged recombinant SARS-CoV-2 Spike S1 protein is a popular research reagent and IVD material to manufacture SARS-CoV-2 antigen and antibody immunoassay kits including rapid test kits and ELISA kits. Please ask for a quote if you need protein mutants or a protein with different modification or components.

Syd Labs also provide custom antibody discovery services to screen more and better antibodies for therapeutic drugs, diagnostic immunoassays, and research reagents:

SARS-CoV-2-specific human neutralizing monoclonal antibody development service: If a client or collaborator provides PBMC from recovered COVID-19 patients or people with SARS-CoV-2 vaccines, Syd Labs can do single B cell sorting and culturing. We can send out about 70 ul supernatant per well (clonal single B cell) for quick screening. Then you tell us which single B cell clone you like. We sequence the antibody VH/VL and produce the recombinant human monoclonal antibody for your confirmation. The efficiency is much higher than antibody cloning and sequencing immediately after single B cell sorting; of course, the total cost is much lower. A few groups have used the single B cell method without culturing after sorting to identify some neutralizing monoclonal antibodies against the SARS-CoV-2 spike protein, especially its S1 RBD. It remains unknown how good they are to become therapeutic drugs. Using the strategy without culturing, one may screen antibodies from hundreds of single B cells after a lot of efforts; however, one may easily screen antibodies from thousands of cultured single B cells.

SARS-CoV-2 Spike S1 rabbit monoclonal antibody development service: We immunized rabbits with SARS-CoV-2 S protein S1 subunit, then did single B cell sorting and culturing and identified a number of anti-S1 or RBD-specific rabbit monoclonal antibodies. We measured affinity and pairing possibility of some recombinant rabbit mAbs, which can be used for clinical diagnostics to detect the SARS-CoV-2 antigen. Some rabbit mAbs even have the neutralization function to block the RBD-ACE2 interaction, which is critical for antibody discovery for therapeutics.

SARS-CoV-2 spike S1 VHH nanobody development service: Syd Labs immunized alpaca with SARS-CoV-2 S protein S1 subunit and Nucleocapsid protein, then used two strategies to screen alpaca nanobodies: single B cell sorting and culturing, and VHH phage display. The spike nanobodies may be good candidates for therapeutic drugs alone or by combination with other proteins or antibodies; the nucleocapsid nanobodies may be used for diagnostic test kits.

To know more about our human, rabbit, and alpaca (camelid) monoclonal antibody development services, please visit:

Human monoclonal antibody production services
Rabbit monoclonal antibody development services
Single B cell antibody cloning and sequencing services
Camelid monoclonal antibody production services

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