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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant SARS-CoV-2 S protein S1 subunit-His protein, biotinylated His-tag and Fc-fused S protein S1 subunits, SARS-CoV-2 S protein S1 antibodies, immunoassay kits, custom antibody and kit development services are available.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Source: Recombinant SARS-CoV-2 Spike S1 protein (S protein S1 subunit, Gln14-Arg683) was expressed in mammalian cells with a Fc tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 102.6 kDa. Due to glycosylation, the recombinant SARS-CoV-2 S protein S1 subunit migrates to 130-140 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Activity: Immobilized recombinant human ACE2, His Tag at 0.5 ug/ml (100 ul/well). Dose response curve for the recombinant SARS-CoV-2 Spike S1-Fc-Avi protein with the EC50 of 0.1 ug/ml determined by ELISA.
Formulation: The recombinant SARS-CoV-2 Spike S1-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.
Shipping: The recombinant SARS-CoV-2 Spike S1-Fc-Avi protein is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Background
There are two subunits, S1 and S2, in the SARS-CoV-2 spike (S) glycoprotein which plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of virus entry inhibitors, neutralizing antibodies, and vaccines. The S1 subunit, the N-terminal 14–685 amino acids of S protein, contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM). The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit.
Both His-tagged and Fc-fused recombinant SARS-CoV-2 S protein S1 subunits are available with an Avi site, which is for biotinylation. The biotinylated recombinant S protein S1 subunits are also avaible. Please ask for a quote if you need S protein S1 subunit mutants or a S protein S1 subunit with different modification or components.
To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services
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