BP005540: Human Notch 1 Protein with the C-His-Avi Tag
Source: The recombinant Human Notch 1 Protein, (amino acids Ala19-Gln526) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human Notch 1 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human DLL4 protein with the C-hFc tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005623: Biotinylated Human Notch 1 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Notch 1 Protein, (amino acids Ala19-Gln526) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant BBiotinylated Human Notch 1 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human DLL4 protein with the C-hFc tag with the EC50 of 7.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.