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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal recombinant Human Notch 1 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005540: Human Notch 1 Protein with the C-His-Avi Tag
Source: The recombinant Human Notch 1 Protein, (amino acids Ala19-Gln526) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Notch 1 protein with the His-Avi tag at the C-terminus has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Notch 1 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human DLL4 protein with the C-hFc tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Notch 1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Human Notch 1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following Notch Protein :
Biotinylated Human Notch 1 Protein
Biotinylated Human Notch 2 Protein
Biotinylated Human Notch 3 Protein
Human Notch 1 Protein
Human Notch 2 Protein
Human Notch 3 Protein
Mouse Notch 4 Protein
Syd Labs provides the following NOTCH Antibody :
Notch 1 Antibody
NOTCH4 Antibody
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