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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: OKT8.
Isotype: Mouse IgG2a kappa.
Source: The anti-human CD8a monoclonal antibody (clone: OKT8) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: OKT8) specifically binds to human CD8a.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD8 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The recombinant OKT8 antibody binds to the human CD8 protein (cluster of differentiation 8), a type I transmembrane glycoprotein and the co-receptor for the T-cell receptor (TCR). Along with the TCR, the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell-antigen interactions. The CD8 protein is predominantly expressed on the surface of cytotoxic T cells, but can also be found on natural killer cells, cortical thymocytes, and dendritic cells. The CD8 molecule is a marker for cytotoxic T cell population.
Steroid-sensitive mechanism of soluble immune response suppressor production in steroid-responsive nephrotic syndrome
Tomana, M., et al. (1987). J Clin Invest. 1987 Jan;79(1):257-64. doi: 10.1172/JCI112792. PMID: 3793925
The OKT8 antibody (anti-human CD8a) was used to identify and deplete OKT8+ suppressor T cells in serum from patients with steroid-responsive nephrotic syndrome. OKT8 depletion eliminated soluble immune response suppressor (SIRS) activity, confirming its production by OKT8+ cells, which was inhibited by steroids both in vitro and in vivo.
Tags: anti-human CD8a OKT8; anti-human CD8a OKT8 mAb
Circulating activated suppressor T lymphocytes in aplastic anemia
Gascon, P., et al. (1985). N Engl J Med. 1985 Jan 31;312(5):257-65. doi: 10.1056/NEJM198501313120501. PMID: 2981406
The OKT8 antibody (anti-human CD8a) identified activated suppressor T lymphocytes in aplastic anemia patients via flow cytometry. OKT8 depletion restored hematopoiesis in vitro, and in vivo OKT8+ cell counts correlated with disease severity, with targeted treatment improving outcomes in some patients.
Tags: anti-human CD8a OKT8 antibody in vivo; anti-human CD8a OKT8 in animal model
Regulation of normal human blood neutrophilic, macrophagic, and eosinophilic committed stem cell proliferation by autologous blood T lymphocyte subsets
Bagby, G. C. Jr., et al. (1984). Blood. 1984 Feb;63(2):356-63. PMID: 6229296
The OKT8 antibody (anti-human CD8a) identified OKT8+ T lymphocytes that inhibited committed stem cell proliferation in a contact-independent manner. OKT8 depletion enhanced stem cell growth in culture, suggesting a regulatory role for OKT8+ cells in myelopoiesis in vivo.
Tags: anti-human CD8a OKT8 mAb in animal model; anti-human CD8a OKT8 in cancer research
Control of polyclonal immunoglobulin production from human lymphocytes by leukotrienes; leukotriene B4 induces an OKT8 (anti-human CD8a) (+), radiosensitive suppressor cell from resting, human OKT8(-) T cells
Rola-Pleszczynski, M., et al. (1984). J Clin Invest. 1984 May;73(5):1334-40. doi: 10.1172/JCI111338. PMID: 6090503
The OKT8 antibody (anti-human CD8a) identified radiosensitive OKT8+ suppressor cells induced by leukotriene B4 from OKT8- T cells. These cells suppressed B cell differentiation and immunoglobulin production in vivo, with OKT8 used to confirm the suppressor cell phenotype in flow cytometry assays.
Tags: anti-human CD8a OKT8 antibody; bioactivity of anti-human CD8a OKT8
Requirement for OKT8+ suppressor cell proliferation for suppression by human newborn T cells
Hayward, A. R., et al. (1981). Clin Exp Immunol. 1981 Sep;45(3):468-74. PMID: 6461445
The OKT8 antibody (anti-human CD8a) identified increased OKT8+ suppressor cells in PWM-stimulated newborn lymphocyte cultures. OKT8 depletion with complement or 50 microM deoxyguanosine reduced suppressor activity, indicating that OKT8+ cell proliferation is required for suppression, with implications for neonatal tolerance in vivo.
Tags: anti-CD8a clone OKT8; OKT8 anti-human CD8a antibody
For more references about anti-human CD8a antibody (OKT8), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)
Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)
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