Single B cell antibody cloning and sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by Sanger DNA sequencing or next generation sequencing (NGS) has been successfully applied to single B cell antibody technologies.
Custom Ilama and alpaca monoclonal antibody development (VHH, sdAb or nanobody) by Ilama or alpaca immunization and phage display screening.
Production of camel, Ilama or alpaca VHH in E.coli or VHH-Fc in HEK 293 cells.
Fast turnaround: 4-6 months once the antigen is available.