BP005406: Human TNFR2/CD120b/TNFRSF1B Protein with the C-His Tag

Source: The recombinant Human TNFR2/CD120b/TNFRSF1B Protein, (amino acids Leu23-Asp257) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human TNFR2 protein with the C-His tag at 5 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Human TNF alpha protein with the C-His tag with the EC50 of 14.6 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003360: Human TNFR2/CD120b/TNFRSF1B Protein with the C-hFc Tag

Source: The recombinant Human TNFR2/CD120b/TNFRSF1B Protein, (amino acids Leu23-Asp257) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human TNF alpha protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFR2 protein with the C-hFc tag with the EC50 of 7.1 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005559: Human TNFR2/CD120b/TNFRSF1B Protein with the C-mFc Tag

Source: The recombinant Human TNFR2/CD120b/TNFRSF1B Protein, (amino acids Leu23-Asp257) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human TNF alpha protein with the C-mFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFR2 protein with the C-mFc tag with the EC50 of 45.8 ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005162: Human TNFR1/CD120a/TNFRSF1A Protein with the C-His Tag

Source: The recombinant Human TNFR1/CD120a/TNFRSF1A Protein, (amino acids Leu30-Thr211) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human TNFR1 protein with the C-His tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Human TNF alpha protein with the C-His tag with the EC50 of 16.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005161: Human TNFR1/CD120a/TNFRSF1A Protein with the C-hFc Tag

Source: The recombinant Human TNFR1/CD120a/TNFRSF1A Protein, (amino acids Leu30-Thr211) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human TNF alpha protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human TNFR1 protein with the C-hFc tag with the EC50 of 3.8 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003710: Cynomolgus TNFR1/CD120a/TNFRSF1A Protein with the C-His Tag

Source: The recombinant Cynomolgus TNFR1/CD120a/TNFRSF1A Protein, (amino acids Leu30-Thr211) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus TNFR1 protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human TNF alpha protein with the C-His tag with the EC50 of 17.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP000123-CYT-252: Recombinant Mouse Tumor Necrosis Factor α (TNFα) Protein

Source: E. coli-derived.
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.1 ug/ml, corresponding to a Specific Activity of 10,000,000 Units/mg.
Purity: > 97.0% by RP-HPLC and SDS-PAGE.

BP000123-CYT-393: Recombinant Rat Tumor Necrosis Factor-α (TNFα) Protein

Source: E. coli-derived.
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05 ng/ml, corresponding to a Specific Activity of 20 MIU/mg.
Purity: > 95% by SDS-PAGE and RP-HPLC.

BP000123-CYT-405: Recombinant Porcine Tumor Necrosis Factor-α (TNFα) Protein

Source: E. coli-derived.
The ED50 as determined by the cytotoxicity of porcine PK15 cells was found to be 0.001-0.015 ng/ml.
Purity: > 95% by SDS-PAGE and RP-HPLC.

BP000123-CYT-494: Recombinant Human Tumor Necrosis Factor-α (TNFα) Protein, His Tag

Source: E. coli-derived.
Purity: > 95% by SDS-PAGE and RP-HPLC.

BP000123-CYT-114: Recombinant Human Tumor Necrosis Factor-Alpha (TNFα) Protein, HEK

Source: HEK-derived.
The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D and is typically 0.05-0.5 ng/ml.
Purity: > 95% as obsereved by SDS-PAGE.

BP003673: Cynomolgus TNF alpha Protein with the N-His Tag

Source: The recombinant Cynomolgus TNF alpha Protein, (amino acids Val77-Leu233) fused with a His tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus TNF alpha protein with the N-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFR1 protein with the N-hFc tag with the EC50 of 1.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP000123-CYT-140: Recombinant Canine Tumor Necrosis Factor-Alpha (TNFα) Protein

Source: E. coli-derived.
The Specific Activity is >3.3?05 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Purity: > 95.0% by RP-HPLC and SDS-PAGE.