BP004746: Human RANKL/TNFSF11/CD254 Protein with No Tag
Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A, hFc tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP004797: Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag
Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human RANKL protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A protein with the N-hFc tag with the EC50 of 17.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003873: Human TNFRSF11A/Rank Protein with the C-hFc Tag
Source: The recombinant Human TNFRSF11A/Rank Protein, (amino acids Ile30-Pro212) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human RANKL protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A protein with the C-hFc tag with the EC50 of 17.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003857: Human TNFRSF11B Protein with the C-His Tag
Source: The recombinant Human TNFRSF11B Protein, (amino acids Glu22-Leu401) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human RANKL protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11B protein with the C-His tag with the EC50 of 16.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004051: Mouse RANKL/TNFSF11/CD254 Protein with the C-His Tag
Source: The recombinant Mouse RANKL/TNFSF11/CD254 Protein, (amino acids Tyr70-Asp316) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse RANKL protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse TNFRSF11A protein with the C-hFc tag with the EC50 of 8.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003872: Mouse TNFRSF11A/Rank Protein with the C-hFc Tag
Source: The recombinant Mouse TNFRSF11A/Rank Protein, (amino acids Val31-Ser214) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse RANKL protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse TNFRSF11A protein with the C-hFc tag with the EC50 of 8.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000050-GD13: Recombinant Human RANKL Protein
Source:E. coli-derived.
The RANKL / TNFSF11 / TRANCE / OPGL / CD254 activity is calculated by its ability to induce osteoclast differentiation on RAW 264.7 mouse macrophages. The ED50 for this effect is 2-12 ng/ml.
Endotoxin Level: <1.0 EU per ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE and SEC-HPLC.BP000173-CYT-320: Recombinant Mouse RANK Ligand Soluble
Source: E. coli-derived.
A single, non-glycosylated, polypeptide chain containing 174 amino acids and having a total molecular mass of 19.9 kDa.
Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 5.0-10.0 ug/ml, corresponding to a specific activity of 100,000-200,000 Units/mg.
Purity > 95%, by SDS-PAGE and RP-HPLC.BP000173-CYT-334: Recombinant Human RANK Ligand Soluble
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.
The activity as determined by its ability to induce a dose dependant stimulation of IL-8 production in human PBMCs, is 39-51 ug/ml.
Purity > 98%, by SDS-PAGE and RP-HPLC.BP000173-CYT-631: Recombinant Human Soluble RANK Ligand, GST tag
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.
Purity > 95%, by SDS-PAGE.
BP000173-CYT-692: Recombinant Human Soluble RANK Ligand, His Tag
Source: E. coli-derived.
Purity > 80%, by SDS-PAGE.