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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal recombinant Human RANKL/TNFSF11/CD254 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP004746: Human RANKL/TNFSF11/CD254 Protein with No Tag
Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with No Tag, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human RANKL/TNFSF11/CD254 Protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu
Activity: Immobilized recombinant Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A, hFc tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human RANKL/TNFSF11/CD254 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP004797: Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag
Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human RANKL/TNFSF11/CD254 protein with the His-Flag tag at the N-terminus has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human RANKL protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A protein with the N-hFc tag with the EC50 of 17.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human RANKL/TNFSF11/CD254 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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