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Human RANKL Protein

ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2

Catalog No. Product Name Size List Price (US$) Quantity
BP004746 Human RANKL/TNFSF11/CD254 Protein with No Tag 100 ug 375.00
BP004746 Human RANKL/TNFSF11/CD254 Protein with No Tag 500 ug 1250.00
BP004797 Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag 100 ug 315.00
BP004797 Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag 500 ug 1050.00
Description

BP004746: Human RANKL/TNFSF11/CD254 Protein with No Tag

Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with No Tag, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human RANKL/TNFSF11/CD254 Protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu
Activity: Immobilized recombinant Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A, hFc tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human RANKL/TNFSF11/CD254 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP004797: Human RANKL/TNFSF11/CD254 Protein with the N-His-Flag Tag

Source: The recombinant Human RANKL/TNFSF11/CD254 Protein, (amino acids Gly63-Asp244) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human RANKL/TNFSF11/CD254 protein with the His-Flag tag at the N-terminus has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human RANKL protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TNFRSF11A protein with the N-hFc tag with the EC50 of 17.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human RANKL/TNFSF11/CD254 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human RANKL/TNFSF11/CD254 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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