BP003077: Recombinant Human SIRP alpha V8 Protein

Source: mammalian cells.
Purity: > 95% by Bis-Tris PAGE and HPLC.

BP004374: Biotinylated Human SIRP alpha V2/CD172a Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V2/CD172a Protein, (amino acids Glu31-Arg369) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human SIRP alpha V2 protein with the C-His tag with the EC50 of 0.36 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004397: Biotinylated Human SIRP alpha V5 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V5 Protein, (amino acids Glu31-Arg370(S42F)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human SIRP alpha V5 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 5.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004829: Mouse SIRP alpha/CD172a Protein with the C-His Tag

Source: The recombinant Mouse SIRP alpha/CD172a Protein, (amino acids Lys32-Asn373) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse SIRP alpha protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse CD47 protein with the C-hFc tag with the EC50 of 0.26 ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005658: Biotinylated Human SIRP alpha/CD172a Protein with the C-hFc-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-hFc-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human SIRP alpha protein with the C-hFc tag with the EC50 of 0.22 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004965: Biotinylated Human SIRP alpha V4 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V4 Protein, (amino acids Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human SIRP alpha V4 protein with the C-His tag with the EC50 of 0.18 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004962: Human SIRP alpha V6 Protein with the C-His-Avi Tag

Source: The recombinant Human SIRP alpha V6 Protein, (amino acids Glu31-Arg370(S105P)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha V6 protein with the C-His-Avi tag at 5 ug/ml (100 ul/Well). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 0.76 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004396: Biotinylated Human SIRP alpha V6 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V6 Protein, (amino acids Glu31-Arg370(S105P)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human SIRP alpha V6 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 0.21/ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004395: Biotinylated Human SIRP alpha V3 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V3 Protein, (amino acids Glu31-Arg369(H54L)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human SIRP alpha V3 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 11.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP001107-C385: Recombinant Human Signal-Regulatory Protein α?-1 (SIRPA) Protein

Source: HEK 293-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).