PA007638.r2a: In Vivo Grade Recombinant Anti-mouse SIRPA Monoclonal Antibody (Clone: MY-1), Rat IgG2a Kappa

Recombinant Rat IgG2a Monoclonal Antibody.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: MY-1) specifically binds to mouse SIRPA.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse SIRPA protein.

BP005658: Biotinylated Human SIRP alpha/CD172a Protein with the C-hFc-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-hFc-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human SIRP alpha protein with the C-hFc tag with the EC50 of 0.22 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004374: Biotinylated Human SIRP alpha V2/CD172a Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V2/CD172a Protein, (amino acids Glu31-Arg369) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human SIRP alpha V2 protein with the C-His tag with the EC50 of 0.36 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004395: Biotinylated Human SIRP alpha V3 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V3 Protein, (amino acids Glu31-Arg369(H54L)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human SIRP alpha V3 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 11.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004965: Biotinylated Human SIRP alpha V4 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V4 Protein, (amino acids Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human SIRP alpha V4 protein with the C-His tag with the EC50 of 0.18 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004397: Biotinylated Human SIRP alpha V5 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V5 Protein, (amino acids Glu31-Arg370(S42F)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human SIRP alpha V5 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 5.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004396: Biotinylated Human SIRP alpha V6 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human SIRP alpha V6 Protein, (amino acids Glu31-Arg370(S105P)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human SIRP alpha V6 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 0.21/ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004969: Cynomolgus SIRP alpha/CD172a Protein with the C-His Tag

Source: The recombinant Cynomolgus SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus SIRP alpha protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-SIRP alpha Antibody protein with the C-hFc tag with the EC50 of 5.4 ng/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005321: Human SIRP alpha/CD172a Protein with the C-His Tag

Source: The recombinant Human SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 99.1 ng/ml determined by ELISA (QC Test). The affinity constant of 20.6 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005320: Human SIRP alpha/CD172a Protein with the C-hFc Tag

Source: The recombinant Human SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 82.1 ng/ml determined by ELISA (QC Test). The affinity constant of 18.9 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005744: Human SIRP alpha V2/CD172a Protein with the C-His Tag

Source: The recombinant Human SIRP alpha V2/CD172a Protein, (amino acids Glu31-Arg369) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha V2 protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 6.7 ng/ml determined by ELISA (QC Test). The affinity constant of 9.11 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005755: Human SIRP alpha V2/CD172a Protein with the C-hFc Tag

Source: The recombinant Human SIRP alpha V2/CD172a Protein, (amino acids Glu31-Arg369) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-hFc tag at 2 ug/ml (100 ul/Well). Dose response curve for the recombinant Human SIRP alpha V2 protein with the C-hFc tag with the EC50 of 41.9 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.