20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
BP004614: Cynomolgus IgE Protein with the C-His-Avi Tag
Source: The recombinant Cynomolgus IgE Protein, (amino acids Lys208-Lys429) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus IgE protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-IgE Antibody protein with the C-hFc tag with the EC50 of 4.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003820: Human IgE Protein with the C-His-Avi Tag
Source: The recombinant Human IgE Protein, (amino acids Cys209-Lys428) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human IgE protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-IgE Antibody protein with the C-hFc tag with the EC50 of 7.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.PA000304-000101A02: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: Sandwich ELISA;
Reactivity: Human;
Clone No: 1A2;
Isotype: Mouse IgG1.PA000304-000104C03: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: Indirect ELISA, Sandwich ELISA, WBting;
Reactivity: Human;
Clone No: 4C3;
Isotype: Mouse IgG1.PA000304-4C3-1A2: IgE Monoclonal Antibody Pairs
Mouse Monoclonal Antibody;
Applications: Sandwich ELISA;
Reactivity: Human;
Isotype: IgG1.PA000304-Mab-83101: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Reactivity: Human.PA006340-PA1618: IL4R Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB, IHC-P;
Reactivity: Human;
Isotype: Rabbit IgG.C020P: Omalizumab Biosimilar, Human IgE Monoclonal Antibody
Recombinant Humanized IgG1 Monoclonal Antibody.
Specificity/Sensitivity: The monoclonal antibody Omalizumab specifically binds to the human immunoglobulin E (IgE) free in the blood and interstitial fluid and to membrane-bound form of IgE (mIgE) on the surface of mIgE-expressing B lymphocytes. It does not bind to IgE that is already bound by the high affinity IgE receptor (FcεRI) on the surface of mast cells, basophils, and antigen-presenting dendritic cells.
Applications: Functional assay, neutralization, blocking, ELISA.C020: Xolair Biosimilar Stable Cell Line (CHO)
The yield of Xolair biosimilar from the CHO stable cell line was above 3.0 g/L in a 3 liter manufacturing.