- IA000059-W-9812
1. Money Saving: Usually with 30-50% less antibody we get good results, please see the Figs below.
2, Time saving: 30 minutes to 90 minutes protocol available. BP003567: Human LIF Protein with No Tag
Source: The recombinant Human LIF Protein, (amino acids Ser23-Phe202) fused with No Tag, was produced in E.coli cells.
Activity: Immobilized Human LIF, No tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human LIF R, His tag with the EC50 of 0.30 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005281: Human LIF Protein with No Tag
Source: The recombinant Human LIF Protein, (amino acids Ser23-Phe202) fused with No Tag, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human LIF at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human LIF R, hFc tag with the EC50 of 55.9 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004589: Human LIF Protein with the C-hFc Tag
Source: The recombinant Human LIF Protein, (amino acids Ser23-Phe202) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004555: Human LIF R/CD118 Protein with the C-His Tag
Source: The recombinant Human LIF R/CD118 Protein, (amino acids Gln45-Ser833) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human LIF protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human LIF R protein with the C-His tag with the EC50 of 0.30 ug/ml determined by ELISA (QC Test). The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005781: Human LIF R/CD118 Protein with the C-hFc Tag
Source: The recombinant Human LIF R/CD118 Protein, (amino acids Gln45-Ser833) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human LIF protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human LIF R protein with the C-hFc tag with the EC50 of 66.8 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP004029: Human MIF Protein with the C-His Tag
Source: The recombinant Human MIF Protein, (amino acids Pro2-Ala115) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP002670-PRO-375: Recombinant Human Intrinsic Factor (IF) protein
Source: Sf9 Insect Cells-derived.
Biological Activity: Standard ELISA test (checker-board analysis of positive/negative sera panels), immunodot with anti-IF autoantibody positive patient sera.
Can be used for solid ELISA and fluid phase diagnostic assays, 0.25-0.65 ug/ml.
Suitable for biotinylation and iodination.
Purity: > 95% by RP-HPLC and SDS-PAGE.
BP000092-C017: Recombinant Human Leukemia Inhibitory Factor (LIF) Protein
Source: E. coli-derived.
The ED50 was determined by the M1 cell differentiation assay is < 0.01 ug/ml, corresponding to a specific activity of 1.0 x 108 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).BP000092-CYT-001: Recombinant Human Leukemia Inhibitory Factor, GST tag
Source: E. coli-derived.
Purity: > 90% by SDS-PAGE and RP-HPLC.BP000092-CYT-644: Recombinant Human Leukemia Inhibitory Factor (LIF) Protein
Source: E. coli-derived.
The ED50 was determined by the M1 cell differentiation assay is < 0.01 ug/ml, corresponding to a specific activity of 100 MIU/mg.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000092-CYT-645: Recombinant Mouse Leukemia Inhibitory Factor (LIF) Protein
Source: E. coli-derived.
The specific activity is 100 MIU/mg, where 50 U is defined as the amount of murine LIF required to induce differentiation in 50% of the M1 colonies per ml of agar culture.
Purity: > 95% by SDS-PAGE and RP-HPLC.BP000222-CYT-431: Recombinant Human Macrophage Migration Inhibitory Factor, His Tag N-Terminus
Source: E. coli-derived.
Purity > 95%, by SDS-PAGE and RP-HPLC.