BP005653: Biotinylated Human GM-CSF R alpha Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human GM-CSF R alpha Protein, (amino acids Glu23-Gly320) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-GM-CSF R alpha Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GM-CSF R alpha protein with the C-His tag with the EC50 of 11.6 ng/ml determined by ELISA (QC Test). The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.PA000575-PA1456: GM-CSF Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB;
Reactivity: Human;
Isotype: Rabbit IgG.PA000575-Mab-C40090A: GM-CSF Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: ELISA;
Reactivity: Human, Others not tested;
Clone No: 29 (formerly 19);
Isotype: IgG1.PA000575-ANT-129: GM-CSF Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: WB, ELISA, IP;
Reactivity: Human;
Clone: NYRhGMCSF;
Isotype: Mouse IgG.BP000176-C003: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1 ng/ml, corresponding to a Specific Activity of 1.0 x 107 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).BP000176-C040: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein
Source: p.pichia-derived.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.2 ng/ml, corresponding to a Specific Activity of 5.0 x 106 IU/ mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).BP000176-CYT-221: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11,100,000 IU/mg.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000176-CYT-324: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein, Pichia
Source:Pichia Pastoris-derived.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.183 ng/ml, corresponding to a Specific Activity of 5.5 MIU/mg.
Purity: > 97% by SDS-PAGE and RP-HPLC.BP000176-CYT-222: Recombinant Mouse Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Met-Ala-Pro-Thr-Arg.
The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.
Purity: > 98% by SDS-PAGE and RP-HPLC.BP000176-CYT-395: Recombinant Rat Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein
Source: E. coli-derived.
The ED50 is 0.001-0.01 ng/ml corresponding to a specific activity of 100-1,000 MIU/mg, determined by the dose dependent proliferation of murine MC/9 cells.
Purity: > 95% by SDS-PAGE and RP-HPLC.