20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
BS046B:
Gene synthesis of the variable regions of the recombinant IgG antibodies;
Molecular construction of the recombinant IgG antibodies;
0.04 Liter transient production of the recombinant IgG antibodies in CHO cells;
Protein A purification.PA000256-030409B06: Anti-Human IgG Antibody
Mouse Monoclonal Antibody;
Applications: Indirect ELISA;
Reactivity: Human;
Clone No: KT15;
Isotype: Mouse IgG2a.PA000256-P-731: Rabbit anti-Human IgG Antibody (Biotin)
Rabbit Polyclonal Antibody;
Applications: ELISA, WB, immunohistochemistry and I.P;
Reactivity: Human.PA005183-ANT-190: GST AfP Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: ELISA;
Isotype: Rabbit Ig.Single B cell antibody sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by next generation sequencing (NGS) including nanopore sequencing has been successfully applied to single B cell antibody technologies.