100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
- Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
- Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
- Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.PA000304-000101A02: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: Sandwich ELISA;
Reactivity: Human;
Clone No: 1A2;
Isotype: Mouse IgG1.PA000304-000104C03: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: Indirect ELISA, Sandwich ELISA, WBting;
Reactivity: Human;
Clone No: 4C3;
Isotype: Mouse IgG1.PA000304-4C3-1A2: IgE Monoclonal Antibody Pairs
Mouse Monoclonal Antibody;
Applications: Sandwich ELISA;
Reactivity: Human;
Isotype: IgG1.PA005142-MI021: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: EIA;
Reactivity: Human;
Clone: 1E8E12;
Isotype: IgG1.PA005142-MI022: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: EIA;
Reactivity: Human;
Clone: 4F61E10;
Isotype: IgG1.PA006340-PA1618: IL4R Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB, IHC-P;
Reactivity: Human;
Isotype: Rabbit IgG.PA000304-Mab-83101: IgE Monoclonal Antibody
Mouse Monoclonal Antibody;
Reactivity: Human.C020: Xolair Biosimilar Stable Cell Line (CHO)
The yield of Xolair biosimilar from the CHO stable cell line was above 3.0 g/L in a 3 liter manufacturing.
C020P: Omalizumab Biosimilar, Human IgE Monoclonal Antibody
Recombinant Humanized IgG1 Monoclonal Antibody.
Specificity/Sensitivity: The monoclonal antibody Omalizumab specifically binds to the human immunoglobulin E (IgE) free in the blood and interstitial fluid and to membrane-bound form of IgE (mIgE) on the surface of mIgE-expressing B lymphocytes. It does not bind to IgE that is already bound by the high affinity IgE receptor (FcεRI) on the surface of mast cells, basophils, and antigen-presenting dendritic cells.
Applications: Functional assay, neutralization, blocking, ELISA.