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Conditions of optimal recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP005695: Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-His Tag
Source: The recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein, (amino acids Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta protein with the His tag at the C-terminus has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD3E&CD3D at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant OKT3 protein with the C-mFc tag with the EC50 of 18.9 ng/ml determined by ELISA (QC Test). The affinity constant of 0.36 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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