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Conditions of optimal recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP003854: Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-His Tag
Source: The recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein, (amino acids Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta protein with the His tag at the C-terminus has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Anti-CD3 Antibody protein with the C-His tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human CD3E&CD3D protein with the C-His tag with the EC50 of 0.72 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005618: Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein with the C-hFc-Avi Tag
Source: The recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein, (amino acids Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta protein with the hFc-Avi tag at the C-terminus has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Biotinylated Human CD3E&CD3D protein with the C-hFc-Avi tag at 1 ug/ml (100 ul/Well) on streptavidin (5 ug/ml) precoated plate. Dose response curve for the recombinant OKT3 protein with the C-mFc tag with the EC50 of 42.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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