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Conditions of optimal recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein performance should be determined experimentally by the investigator.
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BP003430: Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein with the C-His-Flag Tag
Source: The recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein, (amino acids Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)) fused with a His-Flag tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma protein with the His-Flag tag at the C-terminus has a predicted MW of 11.93 kDa (CD3E) and 11.52 kDa (CD3G). Due to glycosylation, the protein migrates to 13-23 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Cynomolgus CD3E&CD3G protein with the C-His-Flag tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-CD3 Antibody protein with the C-hFc tag with the EC50 of 5.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005433: Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein with the C-hFc Tag
Source: The recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein, (amino acids Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma protein with the hFc tag at the C-terminus has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Cynomolgus CD3E&CD3G protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Anti-CD3 Antibody protein with the C-hFc tag with EC50 of 20.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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