Anti-Mouse CD19 Monoclonal Antibodies for Flow Cytometry

PA007364: Non-conjugated Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)

Recombinant  rat IgG2a Monoclonal Antibody. 
Clone: 1D3.
Isotype:  Rat IgG2a kappa.
Source: The recombinant anti-mouse CD19 monoclonal antibody (clone: 1D3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 1D3 antibody specifically binds to the mouse CD19 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007365: Biotin Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)

Recombinant rat IgG2a Monoclonal Antibody with site-directed biotin conjugation through the Avi tag.
Clone: 1D3.
Isotype: Rat IgG2a kappa.
Source: The biotin anti-mouse CD19 monoclonal antibody (clone: 1D3) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The biotin recombinant 1D3 antibody specifically binds to the mouse CD19 protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The 1D3 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:

Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
Biotin Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
FITC Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
APC Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
PE Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
Pacific Blue Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
PerCP Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
PE/Cy5 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
PE/Cy7 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
iFluor 488 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
iFluor 549 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
iFluor 594 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
iFluor 647 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
iFluor 700 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
APC/Cy7 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)
PerCP/Cy5.5 Recombinant Anti-mouse CD19 Monoclonal Antibody (Clone: 1D3)

Background:

The 1D3 antibody binds to the mouse CD19 protein, a transmembrane protein expressed in all B lineage cells including mouse plasma cells. CD19 plays two major roles in mouse B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of anti-mouse CD19 antibody (1D3)

Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.
Muller N, et al. (2022). Mol Ther Methods Clin Dev. 2022 Jul 8;26:131-141. doi: 10.1016/j.omtm.2022.05.013. PMID: 35755944
Cells were stained with FITC-conjugated anti-CD19 mAb (clone 1D3) and analyzed by flow cytometry to monitor B cell depletion. The depletion efficiency was >95% in peripheral blood as determined by flow cytometry using 1D3 antibody. Peripheral blood was collected and stained with anti-CD19 (1D3) and anti-CD3 antibodies. Flow cytometry was performed on a FACSCalibur using CellQuest software. B cell depletion was confirmed by the absence of CD19+ cells in flow analysis.
Tags: anti-mouse CD19 1D3; anti-mouse CD19 1D3 for flow cytometry

Therapeutic efficacy of anti-CD19 CAR-T cells in a mouse model of systemic lupus erythematosus.
Ma J, et al. (2020). Cell Mol Immunol. 2021 Aug;18(8):1896-1903. doi: 10.1038/s41423-020-0472-1. PMID: 32472023
For flow cytometry, cells were stained with anti-CD19 (clone 1D3) antibody to identify B cells. The percentage of CD19+ B cells was significantly reduced in treated mice. Spleen cells were isolated and stained with 1D3 antibody for CD19 expression. Data were acquired on a BD FACSCanto II flow cytometer. Treatment led to long-term B cell depletion as measured by flow.
Tags: anti-mouse CD19 1D3 in cancer research; anti-mouse CD19 1D3 mAb in cancer research

Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells.
Kochenderfer JF, et al. (2010). Blood. 2010 Nov 11;116(19):3875-86. doi: 10.1182/blood-2010-05-285254. PMID: 20631379
B cells were identified by staining with PE-conjugated anti-CD19 (clone 1D3) antibody. Flow cytometry analysis showed eradication of CD19+ cells in lymphoma. The antibody 1D3 was used to gate B cell population in bone marrow. Peripheral blood B cells were monitored weekly using 1D3. CAR-T cells specifically targeted CD19+ cells as confirmed by flow.
Tags: anti-mouse CD19 1D3 mAb in mouse tumor model; bioactivity of anti-mouse CD19 1D3

Self-renewal of B-1 lymphocytes is dependent on CD19.
Krop I, et al. (1996). Eur J Immunol. 1996 Jan;26(1):238-42. doi: 10.1002/eji.1830260137. PMID: 8566073
Cells were stained with anti-CD19 mAb (1D3) for flow sorting. Peritoneal cells were analyzed for CD19 expression using 1D3. The antibody 1D3 was used in all flow cytometry experiments for B cell identification. Adoptive transfer experiments used sorted CD19+ cells with 1D3. B-1 cell self-renewal was assessed by limiting dilution analysis after sorting with 1D3.
Tags: anti-mouse CD19 1D3 of low endotoxin; 1D3 mAb for flow cytometry

The signaling activity of murine CD19 is regulated during cell development.
Krop I, et al. (1996). J Immunol. 1996 Jul 1;157(1):48-56. PMID: 8683154
Murine B cells were stained with biotinylated 1D3 anti-CD19 mAb followed by streptavidin-PE. Flow cytometry was used to assess CD19 expression levels during development. The 1D3 clone was specific for murine CD19. Developmental stages were gated based on 1D3 staining intensity. Signaling was correlated with CD19 surface density measured by 1D3.
Tags: 1D3 antibody for flow cytometry; function of 1D3 mAb

For more references about anti-mouse CD19 antibody (1D3), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)