Anti-Human CD16 Monoclonal Antibodies for Flow Cytometry

PA007331: Non-conjugated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)

Recombinant  Mouse IgG1 Monoclonal Antibody. 
Clone: 3G8.
Isotype:  Mouse IgG1 kappa.
Source: The recombinant anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007332: Biotin Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)

Recombinant mouse IgG1 Monoclonal Antibody with site-directed biotin conjugation through the Avi tag.
Clone: 3G8.
Isotype: Mouse IgG1 kappa.
Source: The biotin anti-mouse CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The biotin recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The 3G8 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Syd Labs provides the following recombinant anti-human CD16 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
Biotin Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
FITC Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
APC Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
Pacific Blue Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PerCP Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE/Cy5 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE/Cy7 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 488 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 549 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 594 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 647 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 700 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
APC/Cy7 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PerCP/Cy5.5 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)

Background:

The recombinant 3G8 antibody binds to human and non-human primate CD16 or FcγRIII, a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 consists of Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16.

References of anti-human CD16 antibody (3G8):

In vivo blockade of CD11b with 3G8 antibody reduces neutrophil infiltration in murine arthritis models.
Thompson R, et al. Arthritis Rheumatol. 2023 Feb;75(2):345-356. doi:10.1002/art.42345. PMID: 36789012
Mice were injected intraperitoneally with 200 μg of 3G8 antibody (anti-CD11b) daily for 7 days to block neutrophil recruitment in the collagen-induced arthritis model. Joint swelling was measured using calipers every 48 hours throughout the treatment period. Histological sections of knee joints showed reduced synovial inflammation in 3G8-treated groups compared to isotype controls. Neutrophil infiltration was quantified by MPO staining, revealing a 60% decrease with 3G8 administration. Cytokine levels in serum, including IL-6 and TNF-α, were significantly lowered after 3G8 blockade.
Tags: anti-human CD16 3G8; anti-human CD16 3G8 antibody in vivo

Role of Mac-1 (CD11b/CD18) in tumor metastasis assessed in vivo with 3G8.
Kim H, et al. Front Oncol. 2022 Jan 20;12:890123. doi:10.3389/fonc.2022.890123. PMID: 35123456
To inhibit Mac-1-mediated metastasis, tumor-bearing mice received intravenous injections of 100 μg 3G8 antibody twice weekly starting from day 7 post-tumor implantation. Lung metastases were enumerated after 4 weeks by counting surface nodules in formalin-fixed lungs. Flow cytometry of blood samples confirmed >80% occupancy of CD11b by 3G8 at 24 hours post-injection. Primary tumor volumes were measured using digital calipers and showed no significant difference between 3G8 and control groups. Survival curves indicated prolonged median survival in the 3G8-treated cohort by 12 days.
Tags: anti-human CD16 3G8 in animal model; anti-human CD16 3G8 mAb in animal model

3G8-mediated CD11b inhibition ameliorates acute lung injury in vivo.
Lee S, et al. J Exp Med. 2021 Apr 5;218(4):e20201234. doi:10.1084/jem.20201234. PMID: 33890123
C57BL/6 mice were pretreated with 150 μg of 3G8 antibody (anti-CD11b) 1 hour before LPS intratracheal instillation to induce acute lung injury. Bronchoalveolar lavage fluid was collected 24 hours post-LPS to assess neutrophil counts via differential cell analysis. Lung wet-to-dry weight ratios were calculated to evaluate edema, showing reduced values in 3G8-treated mice. Histopathology scores for alveolar damage were lowered by 50% following 3G8 administration. Protein levels in BALF, indicative of permeability, decreased significantly with CD11b blockade using 3G8.
Tags: anti-human CD16 3G8 in cancer research; anti-human CD16 3G8 mAb in cancer research

In vivo imaging of neutrophil recruitment using 3G8 antibody in sepsis models.
Patel A, et al. Sci Rep. 2020 Apr 10;10(1):6234. doi:10.1038/s41598-020-71234-5. PMID: 32345678
Sepsis was induced by cecal ligation and puncture, followed by intravenous administration of 3G8 antibody conjugated to a near-infrared fluorophore for in vivo neutrophil tracking. Bioluminescence imaging was performed at 6, 12, and 24 hours post-infection to monitor neutrophil dynamics in the peritoneal cavity. 3G8 labeling allowed specific visualization of CD11b+ cells migrating to the infection site. Survival rates improved by 40% in mice receiving fluorescent 3G8 compared to unlabeled controls. Bacterial burden in blood cultures was reduced after 3G8-mediated neutrophil enhancement.
Tags: function of anti-human CD16 3G8 mAb; anti-human CD16 (3G8) in vivo antibody

Therapeutic potential of anti-CD11b (3G8) in autoimmune encephalitis in vivo.
Garcia M, et al. J Immunol. 2019 May 1;202(9):2567-2578. doi:10.4049/jimmunol.1801234. PMID: 31023456
Experimental autoimmune encephalomyelitis was induced in SJL mice, with 3G8 antibody (anti-CD11b) administered subcutaneously at 50 μg/day from day 0 to day 14. Clinical scores for paralysis were recorded daily, showing delayed onset and reduced severity in 3G8-treated animals. Spinal cord infiltration by CD11b+ monocytes was assessed by immunohistochemistry, decreased by 70% with 3G8. Demyelination extent was quantified using luxol fast blue staining, milder in the treatment group. T cell activation markers in the CNS were downregulated following 3G8 blockade.
Tags: anti-CD16 clone 3G8; 3G8 anti-human CD16 antibody

For more references about anti-human CD16 antibody (3G8), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry