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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human CD16 Monoclonal Antibodies for Flow Cytometry

Anti-Human CD16 Monoclonal Antibodies for Flow Cytometry

cluster of differentiation 16, Fc gamma RIIIA and Fc gamma RIIIB

Catalog No. Product Name Size List Price (US$) Quantity
PA007331.m1 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Mouse IgG1 Kappa 50 ug 65.00
PA007331.m1 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Mouse IgG1 Kappa 200 ug 130.00
PA007331.h1Fs Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa 50 ug 130.00
PA007331.h1Fs Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa 200 ug 260.00
PA007332.h1Fs-b Biotinylated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa 50 ug 95.00
PA007332.h1Fs-b Biotinylated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa 200 ug 190.00
Description

Background of Anti-Human CD16 Monoclonal Antibody (Clone: 3G8)

The recombinant 3G8 antibody binds to human and non-human primate CD16 or FcγRIII, a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 consists of Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16.

PA007331.m1: Non-conjugated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Mouse IgG1 kappa

Recombinant  Mouse IgG1 Monoclonal Antibody. 
Clone: 3G8.
Isotype:  Mouse IgG1 kappa.
Source: The recombinant anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007331.h1Fs: Non-conjugated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 3G8.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The recombinant anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007332.h1Fs-b: Biotinylated Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain. 
Clone: 3G8.
Isotype:  Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated recombinant anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 3G8 antibody specifically binds to human and non-human primate CD16.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 3G8 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Syd Labs provides the following recombinant anti-human CD16 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
Biotin Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
FITC Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
APC Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
Pacific Blue Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PerCP Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE/Cy5 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PE/Cy7 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 488 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 549 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 594 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 647 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
iFluor 700 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
APC/Cy7 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PerCP/Cy5.5 Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8)

References of Anti-Human CD16 Monoclonal Antibody (Clone: 3G8)

Comparison of the different anti-CD16 antibody clones in the activation and expansion of peripheral blood NK cells.
Kwon, et al. PLoS One. 2023 Jun 9;18(6):e0286915. doi: 10.1371/journal.pone.0286915. PMID: 37294741; PMCID: PMC10258201
Natural killer (NK) cells play an essential role in cancer immunotherapy, and their activation often relies on CD16-mediated signaling pathways. ... In this study, we systematically compared the downstream functional outcomes of using different anti-CD16 antibody clones, including 3G8, DJ130c, and KD1, on human peripheral blood NK cells. ... Our results show that clone 3G8 induced the most robust NK cell proliferation, degranulation, and inflammatory cytokine secretion compared to other candidate clones.
(Tags: anti-human CD16 3G8 mAb; CD16 clone 3G8 NK cell activation)
Factor VIII moiety of recombinant Factor VIII Fc fusion protein impacts Fc effector function and CD16+ NK cell activation.
Ochiel, et al. Front Immunol. 2024 Apr 9;15:1356391. doi: 10.3389/fimmu.2024.1356391. PMID: 38711484; PMCID: PMC11035769
Recombinant Factor VIII Fc fusion proteins (rFVIIIFc) are widely used therapeutic agents, yet the impact of the large FVIII moiety on Fc-mediated effector functions remains poorly understood. ... Here, we investigated how the steric presence of the FVIII moiety modulates Fc binding to Fc?RIIIa (CD16) and subsequent natural killer (NK) cell activation. ... Using the CD16-blocking monoclonal antibody clone 3G8, we confirmed that rFVIIIFc interacts directly with CD16+ NK cells to trigger downstream intracellular signaling and effector cytokine release.
(Tags: recombinant anti-human CD16; 3G8 antibody ADCC assay)
Multiplex interrogation of the NK cell signalome reveals global downregulation of CD16 signaling during lentivirus infection through an IL-18/ADAM17-dependent mechanism.
Sachan, et al. PLoS Pathog. 2023 Sep 11;19(9):e1011617. doi: 10.1371/journal.ppat.1011617. PMID: 37695786; PMCID: PMC10503717
Chronic lentivirus infections severely impair natural killer (NK) cell functionality, yet the precise intracellular signaling defects remain incompletely mapped. ... We utilized single-cell multiplexed signaling assays to profile downstream targets of the CD16 receptor during active viral infection. ... Our findings demonstrate that an IL-18 and ADAM17-dependent pathway drives the shedding of CD16, which was validated using clone 3G8 to track and block functional surface CD16 expression.
(Tags: anti-CD16 clone 3G8 flow cytometry; anti-human CD16 3G8 in vitro)
NK Cytotoxicity Mediated by NK-92 Cell Lines Expressing Combinations of Two Allelic Variants for FCGR3.
Gomez-Milla, et al. Int J Mol Sci. 2024 Jul 12;25(14):7621. doi: 10.3390/ijms25147621. PMID: 39062864; PMCID: PMC11270249
The low-affinity IgG receptor Fc?RIIIa (CD16) displays genetic polymorphisms, particularly the F158V variation, which influences antibody binding affinity and clinical responses to therapeutic antibodies. ... To dissect these differences, we engineered NK-92 cell lines to express single or combined allelic variants of the FCGR3A gene. ... Functional characterization using the anti-CD16 monoclonal antibody clone 3G8 demonstrated distinct differences in receptor trigger thresholds and downstream killing dynamics between the high-affinity and low-affinity variants.
(Tags: FCGR3A antibody 3G8; anti-human CD16 receptor blocking)
Complement activation induces excessive T cell cytotoxicity in severe COVID-19.
Georg, et al. Cell. 2022 Jan 20;185(2):292-312.e25. doi: 10.1016/j.cell.2021.12.019. PMID: 34968393; PMCID: PMC8712270
Severe COVID-19 is characterized by profound immune dysregulation, hyperinflammation, and progressive lung damage. ... We discovered that complement activation in severe patients drives ectopic upregulation of the low-affinity IgG receptor CD16 (Fc?RIII) on T cells, rendering them highly cytotoxic. ... Blockade of this aberrant CD16 expression on T cells using the 3G8 monoclonal antibody significantly reduced immune-complex-mediated vascular injury, highlighting CD16 as a potential therapeutic target.
(Tags: anti-human CD16 3G8 functional grade; 3G8 clone NK cell depletion)
For more technical references or data sheets regarding the Recombinant Anti-Human CD16 Monoclonal Antibody (Clone: 3G8) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2) for flow cytometry

Related Links
Anti-human CD16 Monoclonal Antibody (Clone: 3G8)
PerCP/Cy5.5 Anti-human CD16 3G8 Antibody for Flow Cytometry, Mouse IgG1 kappa
APC/Cy7 Anti-human CD16 3G8 Antibody for Flow Cytometry, Mouse IgG1 kappa
Anti-human CD16 3G8 Antibody, Human IgG1 kappa
APC Anti-human CD16 3G8 Antibody for Flow Cytometry, Mouse IgG1 kappa
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