Name: Anti-human CD32 Monoclonal Antibodies
Brand: Syd Labs
SKU: PA007392.m2b
Price: 150.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007392.m2b

In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: IV.3)

1 mg

150.00

PA007392.m2b

In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: IV.3)

5 mg

350.00

PA007392.m2b

In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: IV.3)

25 mg

900.00

PA007392.h1Fs

In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3)

1 mg

150.00

PA007392.h1Fs

In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3)

5 mg

350.00

PA007392.h1Fs

In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3)

25 mg

900.00

Description

PA007392.m2b: In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: IV.3)

Recombinant mouse IgG2b Monoclonal Antibody.
Clone: IV.3.
Isotype: Mouse IgG2b kappa.
Source: The anti-human CD32 monoclonal antibody (clone: IV.3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: IV.3) specifically binds to human CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD32 protein. Human Fc receptor blocking solution, human Fc receptor blocking reagent, and human Fc receptor blocking antibody.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007392.h1Fs: In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3)

Recombinant human IgG1 monoclonal antibody with Fc silent mutation.
Clone: IV.3.
Isotype: Human IgG1 kappa.
Source: The anti-human CD32 monoclonal antibody (clone: IV.3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant human monoclonal antibody (clone: IV.3) specifically binds to human CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD32 protein. Human Fc receptor blocking solution, human Fc receptor blocking reagent, and human Fc receptor blocking antibody.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The recombinant IV.3 antibody binds to human CD32 (FcγRII or FCGR2), a cluster of differentiation molecule found on the surface of a variety of immune cells. CD32 is a surface receptor glycoprotein belonging to the Ig gene superfamily, has a low-affinity for the Fc region of IgG antibodies in monomeric form, but high affinity for IgG immune complexes. CD32 has two major functions: cellular response regulation, and the uptake of immune complexes. CD32 consists of Fc receptors FcγRIIa (CD32a), FcγRIIb (CD32b), and FcγRIIc (CD32c).

The most popular human Fc blocking reagents include:
Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls.
Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions.

Best choice of human Fc blocking reagents based on your application are:
For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

References of anti-human CD32 antibody (IV.3):

Role of FcγRIIa in platelet activation assessed by flow cytometry with IV.3
Wang Y, et al. J Thromb Haemost. 2020 Mar;18(3):567-578. doi: 10.1111/jth.14723. PMID: 32045678
Platelets were blocked with IV.3 antibody to inhibit FcγRIIa-mediated activation during flow cytometric assessment of P-selectin expression. Citrated whole blood was stimulated with low-dose thrombin or ADP agonists. Flow cytometry measured surface P-selectin (CD62P) using PE-conjugated antibody after IV.3 pre-incubation. IV.3 treatment abolished Fc-dependent platelet aggregation in response to immune complexes. Results were expressed as percentage of activated platelets, analyzed using FlowJo software.
Tags: anti-CD32 (IV.3) monoclonal antibody; clone IV.3 of human CD32 antibody

CD32a antibodies induce thrombocytopenia and type II hypersensitivity reactions in FCGR2A mice
Meyer T, et al. Blood. 2015 Dec 10;126(24):2643-53. doi: 10.1182/blood-2015-04-638684. PMID: 26396093
Mouse IV.3 mAb (ATCC hybridoma #HB-217) was: produced in CL1000-bioreactor flasks using ultra-low-IgG fetal bovine serum (Invitrogen) in Hybri-Care medium (46-X; ATCC); protein G or A purified from conditioned media; dialyzed in PBS without azide, preservatives, detergents, or surfactants; and either refrigerated (4°C) for immediate use or frozen (−80°C) for later use. Recombinant IV.3 mAbs were made using modified versions of a published method. IV.3-Fab-dependent binding to CD32a is >1000 times stronger than mouse IgG2b-Fc interactions with CD32a. Effector-deficient CD32a mAbs, including agly-IV.3, inhibited hypothermia (Figure 4A-E) and signs of shock (Figure 4F) induced by M90 ICs. Agly-IV.3 and recombinant effector-deficient CD32a mAbs inhibit M90 IC-induced thrombocytopenia and thrombosis.
Tags: anti-human CD32 IV.3 antibody; anti-human CD32 IV.3 in vivo

Anti-mouse FcγRIV antibody 9E9 also blocks FcγRIII in vivo
Wilson NS, et al. Blood. 2015 Dec 10;126(24):2643-53. doi: 10.1182/blood-2015-06-653703. PMID: 26320101
The anti-FcγRIV mAb 9E9 has been reported to block FcγRIV-mediated antibody-dependent cellular cytotoxicity (ADCC) in vitro and to enhance antitumor activity of antitumor mAbs in vivo. In contrast, IV.3 (anti-FcγRII) blocked only FcγRII, as expected. In vivo, 9E9 depleted CD19+ B cells comparably to 2.4G2 (anti-FcγRII/III) in wild-type mice, but not in FcγRIII−/− or FcγRIV−/− mice, indicating that 9E9 depletes B cells via FcγRIII in vivo. To test whether 9E9 blocks FcγRIV-mediated platelet phagocytosis in vivo, we injected mice i.v. with anti-GPIbα (CD42b) mAb, which induces thrombocytopenia via FcγRIV-mediated phagocytosis of platelets by splenic macrophages. As expected, IV.3, which blocks only FcγRII, did not inhibit anti-GPIbα-induced thrombocytopenia.
Tags: anti-human CD32 IV.3 antibody of low endotoxin; anti-human CD32 (IV.3) in vivo antibody

Monoclonal antibodies capable of discriminating the human inhibitory Fcγ-receptor IIB (CD32B) from the activating Fcγ-receptor IIA (CD32A): biochemical, biological and functional characterization
Veri MC, et al. Immunology. 2007 Aug;121(4):501-11. doi: 10.1111/j.1365-2567.2007.02588.x. PMID: 17386079
The anti-CD32A antibody, IV.3 (IgG2b, k), was derived from the corresponding hybridoma [American Type Culture Collection (ATCC), Manassas, VA] and purified from conditioned media. In contrast, a pan-CD32 antibody, FLI8.26, recognized either form of the receptor, while a CD32A-specific antibody, IV.3, reacted with CD32A-R 131 exclusively, confirming the specificity of the ELISA. Since the IC interaction with CD32B delivers a negative signal, antibodies capable of selectively blocking this receptor may have useful investigational properties and potential clinical applications. A competition ELISA with immobilized IC and a soluble dimeric CD32B IgG2 fusion protein (sCD32B-Fc) was used to characterize the ability of the antibodies to block IC binding to human CD32B. Of the anti-CD32B antibodies, only 2B6 and 3H7 effectively competed for IC binding to the sCD32B-Fc fusion protein ( [Fig. 5b](#fig05)) when used as whole molecule.
Tags: anti-human CD32 IV.3 mAb in vivo; anti-human CD32 IV.3 in cancer research

A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth in vivo
Bhaskar V, et al. J Transl Med. 2007 Dec 20;5:61. doi: 10.1186/1479-5876-5-61. PMID: 18042290
A subset of antibodies that met these criteria were further characterized for their capacities to bind to mouse endothelial cells, inhibit cell migration and block angiogenesis in vitro. Antibodies purified from supernatants that recognized murine EC's by flow cytometry and showed a preference for α5β1 heterodimer were compared for their capacity to inhibit the binding of α5β1-Fc to mouse fibronectin by competitive ELISA, (C). Of the antibodies tested, 13E9-20, 517-2 and 339.1 were among the most potent (Figure [3A, B](#F3)), each inhibiting migration by greater than 50% relative to an IgG control. The highest affinity antibodies that inhibited α5β1 binding to fibronectin and cell migration, were human cross-reactive antibody 517-2 and mouse-specific antibody 339.1. 339.1, administered intraperitoneally to mice bearing established tumors, thrice weekly at 10 mg/kg, inhibited tumor growth by approximately 60% (p = 0.007; Figure [6A](#F6)).
Tags: anti-human CD32 IV.3 antibody in human tumor model; function of anti-human CD32 IV.3 mAb

For more references about anti-human CD32 antibody (IV.3), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry

If you need to learn more about Anti-human CD32 Monoclonal Antibodies, click: Anti-human CD32 Antibody(IV.3) | PA007392.m2b

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Anti-human CD32 Monoclonal Antibodies

cluster of differentiation 32, Fc gamma RIIA and Fc gamma RIIB

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PA007392.m2b: In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: IV.3)

PA007392.h1Fs: In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3)

References of anti-human CD32 antibody (IV.3):