Anti-human CD32 Monoclonal Antibodies

PA007392.m2b: In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: IV.3)

Recombinant mouse IgG2b Monoclonal Antibody. 
Clone: IV.3.
Isotype: Mouse IgG2b kappa.
Source: The anti-human CD32 monoclonal antibody (clone: IV.3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: IV.3) specifically binds to human CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD32 protein. Human Fc receptor blocking solution, human Fc receptor blocking reagent, and human Fc receptor blocking antibody.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007392.h1Fs: In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3)

Recombinant human IgG1 monoclonal antibody with Fc silent mutation.
Clone: IV.3.
Isotype: Human IgG1 kappa.
Source: The anti-human CD32 monoclonal antibody (clone: IV.3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant human monoclonal antibody (clone: IV.3) specifically binds to human CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD32 protein. Human Fc receptor blocking solution, human Fc receptor blocking reagent, and human Fc receptor blocking antibody.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The recombinant IV.3 antibody binds to human CD32 (FcγRII or FCGR2), a cluster of differentiation molecule found on the surface of a variety of immune cells. CD32 is a surface receptor glycoprotein belonging to the Ig gene superfamily, has a low-affinity for the Fc region of IgG antibodies in monomeric form, but high affinity for IgG immune complexes. CD32 has two major functions: cellular response regulation, and the uptake of immune complexes. CD32 consists of Fc receptors FcγRIIa (CD32a), FcγRIIb (CD32b), and FcγRIIc (CD32c). 

The most popular human Fc blocking reagents include:
Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls.
Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. 

Best choice of human Fc blocking reagents based on your application are:
For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

References of Anti-human CD32 Monoclonal Antibodies:

Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies.
Scharffenberger, S. C., et al. Cell Rep. 2024 Oct;43(10):114811. doi: 10.1016/j.celrep.2024.114811. PMID: 39383036
Enriched B cells were resuspended in 200μL EasySep buffer and incubated with 10μL rat serum, 10μL Mouse Serum, 10μL mouse-anti-human CD32 (BD), 10μL mouse-anti-human CD23 (BD) and 10μL mouse-anti-human CD16 (BD). Cells were then washed with EasySep buffer and resuspended in 200μL EasySep buffer containing CD19-BV711 at a 1:200 dilution, CD27-PE-Cy7 at a 1:600 dilution, CD14-FITC at a 1:60 dilution, CD3-FITC at a 1:60 dilution, CD20-AF700 at a 1:300 dilution, IgD-PerCP-Cy5.5 at a 1:120 dilution, IgM-BV605 at a 1:120 dilution, Fixable Viability Dye, V500 at a 1:300 dilution, and i) biotinylated murine anti-idiotypic mAbs conjugated to Total SeqC-0960 APC streptavidin, Total SeqC-0959 APC streptavidin, Total SeqC-0958 APC streptavidin, Total SeqC-0957 APC streptavidin, or Total SeqC-0956 APC streptavidin, ii) recombinant anti-idiotypic mAbs labeled with APC and PE (separately labeled pools) using Zenon Human IgG Labeling Kits (ThermoFisher Scientific) murine anti-idiotypic antibodies labeled with Zenon-PE. ...Enriched B cells were resuspended in 200μL EasySep buffer and incubated with 10μL rat serum, 10μL mouse serum, 10μL mouse-anti-human CD32 (BD),10μL mouse-anti-human CD23 (BD) and 10μL mouse-anti-human CD16 (BD). ...Respiratory syncytial virus (RSV) causes lower respiratory tract infections with significant morbidity and mortality at the extremes of age. ...Here, we develop an anti-idiotypic monoclonal antibody (ai-mAb) immunogen that is specific for unmutated VH3-21/VL1-40 B cell receptors (BCRs). ...Respiratory syncytial virus (RSV) is a common seasonal pathogen that generally causes mild respiratory symptoms in adults but can cause serious lower respiratory tract infection in infants and the elderly.
Tags: anti-human CD32 IV.3 antibody; anti-human CD32 IV.3 in vivo

Human blood neutrophils generate ROS through FcγR-signaling to mediate protection against febrile P. falciparum malaria.
Ofori, E. A., et al. Commun Biol. 2023 Jul;6(1):743. doi: 10.1038/s42003-023-05118-0. PMID: 37463969
Before incubating with merozoites, cells were treated with antibodies against FcγRI (CD64), FcγII (CD32), and FcγIII (CD16), as described previously in ref. 10. Cells were treated for 30 min. at 37 °C with 10 μg/ml of anti-human CD64; anti-human CD32; anti-human CD16, or a combination of these to block FcγR-I, II, and III, respectively. ...Blood phagocytes, such as neutrophils and monocytes, generate reactive oxygen species (ROS) as a part of host defense response against infections. ...Plasmodium falciparum merozoites opsonized with naturally occurring IgG triggered both intracellular and extracellular ROS generation in blood phagocytes, with neutrophils being the main contributors. ...High levels of neutrophil ROS were also associated with protection against febrile malaria in two geographically diverse malaria endemic regions from Ghana and India, stressing the importance of the cooperation between anti-malarial IgG and neutrophils in triggering ROS-mediated parasite killing as a mechanism for naturally acquired immunity against malaria. ...Plasmodium falciparum malaria remains one of the greatest public health challenges, with an estimated 247 million new cases and 619,000 malaria-related deaths worldwide in 2021.
Tags: anti-human CD32 IV.3 mAb in vivo; anti-human CD32 IV.3 in cancer research

For more references about Anti-human CD32 Monoclonal Antibodies please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry

If you need to learn more about Anti-human CD32 Monoclonal Antibodies, click: Anti-human CD32 Antibody(IV.3) | PA007392.m2b