Anti-mouse CD16 / CD32 Monoclonal Antibodies for Flow Cytometry

PA007488.r2b: Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody (Clone: 2.4G2), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody. 
Clone: 2.4G2.
Isotype:  Rat IgG2b Kappa.
Source: The anti-mouse CD16 / CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2.4G2 antibody specifically binds to mouse CD16 and CD32.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007488.h1Fs: Recombinant Anti-mouse CD16 / CD32 Monoclonal Antibody (Clone: 2.4G2), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 2.4G2.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD16 / CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2.4G2 antibody specifically binds to mouse CD16 and CD32.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007717.r2b-b: Biotinylated Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody (Clone: 2.4G2), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 2.4G2.
Isotype:  Rat IgG2b Kappa.
Source: The site-directed biotinylated anti-mouse CD16 / CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 2.4G2 antibody specifically binds to mouse CD16 and CD32.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 2.4G2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The recombinant 2.4G2 antibody binds to mouse CD16 (FcγIII) and CD32 (FcγII) receptor proteins. The major component of some commercial Fc block reagents is the 2.4G2 antibody. The rat, mouse, and human IgG Fc binds to mouse CD64 (FcγI). Thus, the recombinant 2.4G2 antibodies can be used to block non-specific binding of IgGs to mouse Fc receptors in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays. Customers may choose different IgG isotypes for their own applications.  

The most popular mouse Fc blocking reagents include:
Anti-Mouse CD16/CD32 (Clone 2.4G2): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clone X54-5/7.1 is the most widely used anti-mouse FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Normal mouse serum, purified mouse IgGs from mouse serum, or mixes of mouse isotype controls: for general Fc blocking in IHC, and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Purified rat IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. Syd Labs supply various rat IgG isotype controls.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. 

Best choice of mouse Fc blocking reagents based on your application are:
For flow cytometry: anti-mouse CD16/CD32 (Clone 2.4G2).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or rat IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant rat IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

References of Anti-mouse CD16 / CD32 Monoclonal Antibodies for Flow Cytometry:

Single-Cell Multi-Omics Profiling of Immune Cells Isolated from Atherosclerotic Plaques in Male ApoE Knockout Mice Exposed to Arsenic.
Makhani, K., et al. Environ Health Perspect. 2025 Jan;133(1):17007. doi: 10.1289/EHP14285. PMID: 39847705
The cells were then blocked for 30 min at 4°C with anti-mouse CD16/CD32 (BD Biosciences; catalog number 553141; dilution 1:25) to block nonspecific binding of antibodies to Fc receptors, then stained for CD45+ cells, but with an antibody conjugated with BV785 (Biolegend; catalog number 103149, rat anti-mouse; dilution 1:100) for 30 min at 4°C in the dark to distinguish plaque-resident leukocytes from those found in the blood. ...Millions worldwide are exposed to elevated levels of arsenic that significantly increase their risk of developing atherosclerosis, a pathology primarily driven by immune cells. ...While the impact of arsenic on immune cell populations in atherosclerotic plaques has been broadly characterized, cellular heterogeneity is a substantial barrier to in-depth examinations of the cellular dynamics for varying immune cell populations. ...Our data revealed that the transcriptional profile of macrophages from arsenic-exposed mice were significantly different from that of control mice and that differences were subtype specific and associated with cell–cell interaction and cell fates. ...These findings in mice provide insights into how arsenic exposure impacts immune cell types in atherosclerosis, highlighting the importance of considering cellular heterogeneity in studying such effects.
Tags: activity of 2.4G2 antibody; function of 2.4G2 antibody

For more references about Anti-mouse CD16 / CD32 Monoclonal Antibodies for Flow Cytometry please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2) for flow cytometry