BP003206: Human CD47 Protein with No Tag
Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) , was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with No tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 0.24 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005366: Human CD47 Protein with the C-His Tag
Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 82.1 ng/ml determined by ELISA (QC Test). The affinity constant of 18.9 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005367: Human CD47 Protein with the C-hFc Tag
Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 5.0 ng/ml determined by ELISA (QC Test). The affinity constant of 13.8 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP003428: Human CD72 Protein with the N-His Tag
Source: The recombinant Human CD72 Protein, (amino acids Arg117-Asp359) fused with a His tag at the N-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.