BP005730: Human IL-18 Protein with No Tag
Source: The recombinant Human IL-18 Protein, (amino acids Tyr37-Asp193) fused with No Tag, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-18 at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-18BP, hFc tag with the EC50 of 16.9 ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005753: Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein, (amino acids Asp20-Arg335) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Anti-IL-1 Rrp-2 Antibody protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human IL-1 Rrp2 protein with the C-His tag with the EC50 of 46.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP000728-ENZ-286: Recombinant T4 DNA Ligase
Source: Escherichia Colilambda lysogen NM 989-derived.
Incubation of a 50µl reaction containing 13,000 units of T4 DNA Ligase with 1µg of a mixture of single and double-stranded [3H] E. coli DNA (200,000 cpm/ug) for 4 hours at 37C released < 0.3% of the total radioactivity.
One Weiss unit is equivalent to circa 67 cohesive-end ligation units.T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200 mM.