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Conditions of optimal T4 DNA Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
BP000728-ENZ-286: Recombinant T4 DNA Ligase
Source: Escherichia Colilambda lysogen NM 989-derived.
Incubation of a 50µl reaction containing 13,000 units of T4 DNA Ligase with 1µg of a mixture of single and double-stranded [3H] E. coli DNA (200,000 cpm/ug) for 4 hours at 37C released < 0.3% of the total radioactivity.
One Weiss unit is equivalent to circa 67 cohesive-end ligation units.T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200 mM.
Formulation: 50 mM Tris-HCl (pH 7.8 at 25C), 10 mM MgCl2, 10 mM DTT, 1 mM ATP, 25 µg/ml BSA and DNA (0.1 to 1um in 5'termini). Optimal ligation occurs at 16C.
Applications: Cloning of restriction fragments.Joining linkers and adapters to blunt-ended DNA.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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