BP005333: Human DNAM-1/CD226 Protein with the C-His-Avi Tag
Source: The recombinant Human DNAM-1/CD226 Protein, (amino acids Glu19-Asn247) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human DNAM-1 protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human CD155 protein with the C-mFc tag with the EC50 of 0.24 ug/ml determined by ELISA (QC Test). The affinity constant of 0.22 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
BP005048: Human DNAM-1/CD226 Protein with the C-hFc Tag
Source: The recombinant Human DNAM-1/CD226 Protein, (amino acids Glu19-Asn247) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human CD155 protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human DNAM-1 protein with the C-hFc tag with the EC50 of 10.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.BP005049: Biotinylated Human DNAM-1/CD226 Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human DNAM-1/CD226 Protein, (amino acids Glu19-Asn247) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Biotinylated Human DNAM-1 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate(5 ug/ml). Dose response curve for the recombinant Human CD155 protein with the C-mFc tag with the EC50 of 11.0 ng/ml determined by ELISA (QC Test). The affinity constant of 264 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.