BP003734: Mouse IL-2 R alpha/CD25 Protein with the C-His Tag
Source: The recombinant Mouse IL-2 R alpha/CD25 Protein, (amino acids Glu22-Lys236) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse IL-2 R alpha protein with the C-His tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Mouse IL-2 protein with the C-His tag with the EC50 of 0.21 ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
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