- IA000032-1008: Biotin-Capture Beads
The Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical Stability. The modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications. - IA000033-1009
The Mono-Avidin Beads enables mild affinity purification of biotinylated proteins, peptides and other ligands. The mono-avidin molecules immobilized on the beads have a much lower biotin-binding affinity than native avidin thus enabling dissociation of biotinylated molecules using free biotin molecules or low pH buffer. In addition, the Mono-Avidin Beads can be regenerated at least 10 times with no significant loss in binding capacity. - IA000034-1011: Imino-Biotin Beads
The Imino-Biotin Beads enables mild affinity purification of various proteins belonging to the Avidins family. The Imino-Biotin molecules immobilized on the beads are capable of binding Avidins at neutral and basic pH and eluting in under acidic conditions. The Imino-Biotin Beads can be regenerated at least 10 times with no significant loss in binding capacity. - IA000038-B-9007-105K
* Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
* Fast and easy method to biotinylate and determine the amount of labeling
* Label and determine the extent of biotin labeling with one reagent
* Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
* Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. - IA000040-M-1002-010
The high surface area and lower non-specific binding of NanoLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate.
IA000040-M-1003-010
The high surface area and lower non-specific binding of MagnaLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate. - IA000041-M-1002-010
The high surface area and lower non-specific binding of NanoLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate. - IA000044-B-9007-009K
ChromaLink One-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple ? 280 and ? 354 measurement determines the number of biotins per antibody molecule. Highlights:
* Complete Kit, contains sufficient material to biotinylate 100 ug of Antibody
* Fast and easy method to biotinylate and determine the amount of labeling
* Label and determine the extent of biotin labeling with one reagent
* Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
* Nondestructive assay does not destroy biotinylated sample - IA000045-B-9014-009K
The ChromaLink? Digoxigenin One-Shot antibody labeling kit is a convenient, cost-effective way to incorporate a digoxigenin label into a single 100 ug quantity of antibody, all in about 90 minutes. Highlights:
?Complete Kit, contains sufficient material to DIG label 100 ?g of Antibody
?Fast and easy method to DIG label and determine the amount of labeling
?Label and determine the extent of DIG incorporation with one reagent
?Simple calculations based on UV absorbance estimates total DIG incorporation (moles of DIG per mole of protein/peptide
?Nondestructive assay does not destroy DIG labeled sample - IA000053-B-9007-105K
The ChromaLink? Biotin Labeling Kit has everything you need and is faster and easier for labeling and measuring the amount of bound biotin than old traditional HABA:Avidin displacement measurement method. The kit also has simple protocols on a flash drive and water soluble reagents, so it is safe, no organics needed.
Highlights:
? Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
? Fast and easy method to biotinylate and determine the amount of labeling
? Label and determine the extent of biotin labeling with one reagent
? Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
? Nondestructive assay does not destroy biotinylated sample - IA000057-B-1012-010
* Fast and easy method to biotinylate and determine the amount of labeling.
* Label and determine the extent of biotin labeling with one reagent.
* Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
* Nondestructive assay does not destroy biotinylated sample.
* Five aliquots, able to label up to 10 milligrams of protein per aliquot.
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